Organoid cultures from normal and cancer-prone human breast tissues preserve complex epithelial lineages.
Adult
BRCA1 Protein
/ genetics
Breast Neoplasms
Cell Culture Techniques
/ methods
Cell Differentiation
/ genetics
Cell Lineage
/ genetics
Epidermal Growth Factor
/ pharmacology
ErbB Receptors
/ metabolism
Female
Humans
Mammary Glands, Human
/ chemistry
Middle Aged
Organoids
/ chemistry
Single-Cell Analysis
Stem Cells
/ chemistry
Transforming Growth Factor beta
/ antagonists & inhibitors
Young Adult
p38 Mitogen-Activated Protein Kinases
/ antagonists & inhibitors
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
06 04 2020
06 04 2020
Historique:
received:
14
03
2019
accepted:
10
03
2020
entrez:
7
4
2020
pubmed:
7
4
2020
medline:
30
7
2020
Statut:
epublish
Résumé
Recently, organoid technology has been used to generate a large repository of breast cancer organoids. Here we present an extensive evaluation of the ability of organoid culture technology to preserve complex stem/progenitor and differentiated cell types via long-term propagation of normal human mammary tissues. Basal/stem and luminal progenitor cells can differentiate in culture to generate mature basal and luminal cell types, including ER+ cells that have been challenging to maintain in culture. Cells associated with increased cancer risk can also be propagated. Single-cell analyses of matched organoid cultures and native tissues by mass cytometry for 38 markers provide a higher resolution representation of the multiple mammary epithelial cell types in the organoids, and demonstrate that protein expression patterns of the tissue of origin can be preserved in culture. These studies indicate that organoid cultures provide a valuable platform for studies of mammary differentiation, transformation, and breast cancer risk.
Identifiants
pubmed: 32249764
doi: 10.1038/s41467-020-15548-7
pii: 10.1038/s41467-020-15548-7
pmc: PMC7136203
doi:
Substances chimiques
BRCA1 Protein
0
BRCA1 protein, human
0
Transforming Growth Factor beta
0
Epidermal Growth Factor
62229-50-9
EGFR protein, human
EC 2.7.10.1
ErbB Receptors
EC 2.7.10.1
p38 Mitogen-Activated Protein Kinases
EC 2.7.11.24
Types de publication
Evaluation Study
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1711Subventions
Organisme : NCI NIH HHS
ID : P01 CA080111
Pays : United States
Organisme : NCI NIH HHS
ID : P50 CA168504
Pays : United States
Organisme : NCI NIH HHS
ID : R35 CA242428
Pays : United States
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