PACT/PRKRA and p53 regulate transcriptional activity of DMRT1.
Journal
Genetics and molecular biology
ISSN: 1415-4757
Titre abrégé: Genet Mol Biol
Pays: Brazil
ID NLM: 100883590
Informations de publication
Date de publication:
2020
2020
Historique:
received:
23
01
2019
accepted:
08
05
2019
entrez:
7
4
2020
pubmed:
7
4
2020
medline:
7
4
2020
Statut:
epublish
Résumé
The transcription factor DMRT1 (doublesex and mab-3 related transcription factor) has two distinct functions, somatic-cell masculinization and germ-cell development in some vertebrate species, including mouse and the African clawed frog Xenopus laevis. However, its transcriptional regulation remains unclear. We tried to identify DMRT1-interacting proteins from X. laevis testes by immunoprecipitation with an anti-DMRT1 antibody and MS/MS analysis, and selected three proteins, including PACT/PRKRA (Interferon-inducible double-stranded RNA dependent protein kinase activator A) derived from testes. Next, we examined the effects of PACT/PRKRA and/or p53 on the transcriptional activity of DMRT1. In transfected 293T cells, PACT/PRKRA and p53 significantly enhanced and repressed DMRT1-driven luciferase activity, respectively. We also observed that the enhanced activity by PACT/PRKRA was strongly attenuated by p53. Moreover, in situ hybridization analysis of Pact/Prkra mRNA in tadpole gonads indicated high expression in female and male germline stem cells. Taken together, these findings suggest that PACT/PRKRA and p53 might positively and negatively regulate the activity of DMRT1, respectively, for germline stem cell fate.
Identifiants
pubmed: 32251494
pii: S1415-47572020000400702
doi: 10.1590/1678-4685-GMB-2019-0017
pmc: PMC7198010
pii:
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e20190017Références
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