Multiplexed
Journal
Contrast media & molecular imaging
ISSN: 1555-4317
Titre abrégé: Contrast Media Mol Imaging
Pays: England
ID NLM: 101286760
Informations de publication
Date de publication:
2020
2020
Historique:
received:
04
01
2020
accepted:
29
01
2020
entrez:
8
4
2020
pubmed:
8
4
2020
medline:
1
7
2021
Statut:
epublish
Résumé
Gas vesicle nanoparticles (GVs) are gas-containing protein assemblies expressed in bacteria and archaea. Recently, GVs have gained considerable attention for biotechnological applications as genetically encodable contrast agents for MRI and ultrasonography. However, at present, the practical use of GVs is hampered by a lack of robust methodology for their induction into mammalian cells. Here, we demonstrate the genetic reconstitution of protein nanoparticles with characteristic bicone structures similar to natural GVs in a human breast cancer cell line KPL-4 and genetic control of their size and shape through expression of reduced sets of humanized gas vesicle genes cloned into Tol2 transposon vectors, referencing the natural gas vesicle gene clusters of the cyanobacteria
Identifiants
pubmed: 32256252
doi: 10.1155/2020/5425934
pmc: PMC7091528
doi:
Substances chimiques
Bacterial Proteins
0
Xenon Isotopes
0
Xenon-129
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
5425934Informations de copyright
Copyright © 2020 Ryota Mizushima et al.
Déclaration de conflit d'intérêts
RIKEN has a pending patent application already open to the public (WO2018043716A1) regarding this work, in which R. M. and T. M. W. are inventors.
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