Methemoglobin determination by multi-component analysis in coho salmon (

Coho salmon Fish hemoglobin Hemichrome Methemoglobin Multi-component analysis Unstable hemoglobin

Journal

MethodsX
ISSN: 2215-0161
Titre abrégé: MethodsX
Pays: Netherlands
ID NLM: 101639829

Informations de publication

Date de publication:
2020
Historique:
received: 04 01 2020
accepted: 17 02 2020
entrez: 8 4 2020
pubmed: 8 4 2020
medline: 8 4 2020
Statut: epublish

Résumé

Hemoglobin derivatives are often quantified in blood to establish cardio-respiratory status and possible causes of impaired oxygen transport. The derivative known as methemoglobin results from oxidation of hemoglobin and is pathologically relevant because it cannot transport oxygen. In species and individuals possessing unstable methemoglobin, methemoglobin formation leads to rapid hemichrome formation and precipitation. Oxidizing reagents in standard methemoglobin analysis techniques therefore prevent accurate quantification of hemoglobin oxidative degradation products in species possessing unstable hemoglobin. In this study, we demonstrated that individual coho salmon (

Identifiants

pubmed: 32257839
doi: 10.1016/j.mex.2020.100836
pii: S2215-0161(20)30056-X
pii: 100836
pmc: PMC7115134
doi:

Types de publication

Journal Article

Langues

eng

Pagination

100836

Informations de copyright

© 2020 The Authors. Published by Elsevier B.V.

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Auteurs

Stephanie Blair (S)

School of the Environment, Washington State University, Puyallup Research & Extension Center, 2606 Pioneer Ave, Puyallup, WA 98371, USA.

Clyde Barlow (C)

Department of Environmental Studies, The Evergreen State College, 2700 Evergreen Pkwy, Olympia, WA 98505, USA.

Erin Martin (E)

Department of Environmental Studies, The Evergreen State College, 2700 Evergreen Pkwy, Olympia, WA 98505, USA.

Ruth Schumaker (R)

Department of Environmental Studies, The Evergreen State College, 2700 Evergreen Pkwy, Olympia, WA 98505, USA.

Jenifer McIntyre (J)

School of the Environment, Washington State University, Puyallup Research & Extension Center, 2606 Pioneer Ave, Puyallup, WA 98371, USA.

Classifications MeSH