Cloning and Identification of PK15 Cells for Enhanced Replication of Classical Swine Fever Virus.
PK15
cell cloning
classical swine fever virus
high-permissive cells
vaccine production
Journal
Journal of veterinary research
ISSN: 2450-7393
Titre abrégé: J Vet Res
Pays: Poland
ID NLM: 101696630
Informations de publication
Date de publication:
Mar 2020
Mar 2020
Historique:
received:
17
07
2019
accepted:
27
02
2020
entrez:
8
4
2020
pubmed:
8
4
2020
medline:
8
4
2020
Statut:
epublish
Résumé
Classical swine fever virus (CSFV) causes an economically important and highly contagious disease of pigs, leading to economic losses around the world. Attenuated live vaccines with CSFV antigens have played an important role in the prevention and control of the disease. Porcine kidney 15 (PK15) cells have been widely used for the propagation of CSFV, but this cell line is not efficient or homogeneously susceptible to viral infection. To achieve a homogeneous PK15 cell line which enabled high titre replication of CSFV, we used the limiting dilution cell cloning method. We developed two cell clones, PK15-1A6 and PK15-3B1, which respectively have high- and low-permissive phenotypes to CSFV infection. The PK15-1A6, PK15-3B1, and PK15 parent cells showed different characteristics in cell proliferation rate, susceptibility to CSFV infection, and CSFV production. The mean virus titres per millilitre reflected by TCID The PK15-1A6 cell clone is more permissive to CSFV infection than the PK15 parent cells. The screened high-permissive cells will be useful for CSFV propagation and vaccine development
Identifiants
pubmed: 32258794
doi: 10.2478/jvetres-2020-0020
pii: jvetres-2020-0020
pmc: PMC7105985
doi:
Types de publication
Journal Article
Langues
eng
Pagination
9-14Informations de copyright
© 2020 M. Yin et al. published by Sciendo.
Déclaration de conflit d'intérêts
Conflict of Interest Conflict of Interests Statement: The authors declare that there is no conflict of interests regarding the publication of this article.
Références
Virology. 1989 Jul;171(1):18-27
pubmed: 2545029
Viral Immunol. 2018 Jan/Feb;31(1):34-39
pubmed: 28514189
BMC Vet Res. 2018 Apr 24;14(1):138
pubmed: 29699558
Arch Virol. 1993;133(3-4):477-83
pubmed: 8257302
Virology. 2007 Dec 20;369(2):423-30
pubmed: 17889922
Can J Vet Res. 2017 Jul;81(3):186-191
pubmed: 28725108
Antiviral Res. 2014 Apr;104:128-35
pubmed: 24500530
Res Vet Sci. 2003 Dec;75(3):169-78
pubmed: 13129664
Front Microbiol. 2016 Feb 05;7:85
pubmed: 26903966
Virus Res. 2015 Feb 2;197:35-47
pubmed: 25510481
Vet Microbiol. 2014 Aug 6;172(1-2):1-6
pubmed: 24793098
PLoS One. 2015 Oct 02;10(10):e0139457
pubmed: 26431319
J Gen Virol. 1996 Jun;77 ( Pt 6):1311-21
pubmed: 8683221
Infect Genet Evol. 2013 Jul;17:231-8
pubmed: 23608662
Vet Microbiol. 2000 Apr 13;73(2-3):103-19
pubmed: 10785321
Cytotechnology. 2016 Aug;68(4):665-74
pubmed: 25432330
Adv Virus Res. 2015;93:47-160
pubmed: 26111586
Transbound Emerg Dis. 2013 Aug;60(4):370-5
pubmed: 22672483