Hypothermia Effects on Liver and Kidney Oxidative Stress Parameters in an Experimental Model of Sepsis in Rats.
hypothermia
oxidative stress
rats
sepsis
Journal
Journal of veterinary research
ISSN: 2450-7393
Titre abrégé: J Vet Res
Pays: Poland
ID NLM: 101696630
Informations de publication
Date de publication:
Mar 2020
Mar 2020
Historique:
received:
30
05
2019
accepted:
13
01
2020
entrez:
8
4
2020
pubmed:
8
4
2020
medline:
8
4
2020
Statut:
epublish
Résumé
Oxidative stress (OS) seems to be an important mediator of cellular injury, from which sepsis can proceed. Studies have demonstrated the protective effect of controlled hypothermia in sepsis. This study aimed to evaluate its effects on OS parameters in rat hepatic and renal tissue septic after caecal ligation and puncture (CLP). Three groups were appointed (10 rats/group): C (control), SN (sepsis normothermic), and SH (sepsis hypothermic). Ten hours from CLP, the liver and kidneys were harvested and total protein concentration, superoxide dismutase (SOD), glutathione peroxidase (GPx) activities, lipid peroxidation level (malondialdehyde (MDA), carbonylated proteins (2,4-dinitrophenylhydrazine (DNPH), and fatty acid profile were analysed. Sepsis significantly increased SOD and GPx activities in the liver, regardless of the temperature. In renal tissue, GPx activity increased significantly in normothermic conditions and SOD tended to decrease in hypothermic conditions. MDA and DNPH concentrations increase in both tissues after CLP. Hypothermia significantly lowered MDA in the liver but only changed it insignificantly in the kidneys. The DNPH in the liver and kidneys was significantly lower in hypothermic conditions. The unsaturated-to-saturated fatty acids ratio was significantly lower in sepsis, and the fall in temperature raised this ratio. Experimentally induced sepsis in rats enhances OS in the liver and kidneys. The effect of hypothermia on OS indices is dependent on the type of tissue.
Identifiants
pubmed: 32258817
doi: 10.2478/jvetres-2020-0004
pii: jvetres-2020-0004
pmc: PMC7105977
doi:
Types de publication
Journal Article
Langues
eng
Pagination
187-195Informations de copyright
© 2020 B. Sidonia et al. published by Sciendo.
Déclaration de conflit d'intérêts
Conflict of Interest Conflict of Interests Statement: The authors declare that there is no conflict of interests regarding the publication of this article.
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