Adipose-derived stem cells integrate into trabecular meshwork with glaucoma treatment potential.

Adipose Tissue / cytology Adult Stem Cells / cytology Animals Anterior Chamber / cytology Apoptosis Aqueous Humor / physiology Cell Differentiation Cell Movement Cells, Cultured Chemotaxis Dexamethasone / pharmacology Disease Models, Animal Glaucoma / pathology Heterografts Humans In Vitro Techniques Intraocular Pressure / physiology Mice Phagocytosis Regenerative Medicine Trabecular Meshwork / cytology
adipose stem cells; differentiation homing integration trabecular meshwork

Journal

FASEB journal : official publication of the Federation of American Societies for Experimental Biology
ISSN: 1530-6860
Titre abrégé: FASEB J
Pays: United States
ID NLM: 8804484

Informations de publication

Date de publication:
05 2020
Historique:
received: 25 09 2019
revised: 24 02 2020
accepted: 23 03 2020
pubmed: 8 4 2020
medline: 28 1 2021
entrez: 8 4 2020
Statut: ppublish

Résumé

The trabecular meshwork (TM) is an ocular tissue that maintains intraocular pressure (IOP) within a physiologic range. Glaucoma patients have reduced TM cellularity and, frequently, elevated IOP. To establish a stem cell-based approach to restoring TM function and normalizing IOP, human adipose-derived stem cells (ADSCs) were induced to differentiate to TM cells in vitro. These ADSC-TM cells displayed a TM cell-like genotypic profile, became phagocytic, and responded to dexamethasone stimulation, characteristic of TM cells. After transplantation into naive mouse eyes, ADSCs and ADSC-TM cells integrated into the TM tissue, expressed TM cell markers, and maintained normal IOP, outflow facility, and extracellular matrix. Cell migration and affinity results indicated that the chemokine pair CXCR4/SDF1 may play an important role in ADSC-TM cell homing. Our study demonstrates the possibility of applying autologous or allogeneic ADSCs and ADSC-TM cells as a potential treatment to restore TM structure and function in glaucoma.

Identifiants

DOI: 10.1096/fj.201902326R PMID: 32259357 PMC: PMC7254553
pubmed: 32259357
doi: 10.1096/fj.201902326R
pmc: PMC7254553
mid: NIHMS1585331
doi:

Substances chimiques

Dexamethasone 7S5I7G3JQL

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

7160-7177

Subventions

Organisme : NEI NIH HHS
ID : R01 EY025643
Pays : United States
Organisme : NEI NIH HHS
ID : R01 EY025286
Pays : United States
Organisme : NEI NIH HHS
ID : P30 EY008098
Pays : United States

Informations de copyright

© 2020 Federation of American Societies for Experimental Biology.

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Auteurs

Yi Zhou (Y)

Department of Ophthalmology, University of Pittsburgh, Pittsburgh, PA, USA.
Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha, China.

Xiaobo Xia (X)

Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha, China.

Enzhi Yang (E)

Department of Ophthalmology, University of Pittsburgh, Pittsburgh, PA, USA.

Yiwen Wang (Y)

Department of Ophthalmology, University of Pittsburgh, Pittsburgh, PA, USA.
Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha, China.

Kacey G Marra (KG)

Department of Plastic Surgery and Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA.
McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA.

C Ross Ethier (CR)

Department of Biomedical Engineering, Georgia Institute of Technology & Emory University, Atlanta, GA, USA.

Joel S Schuman (JS)

Department of Ophthalmology, New York University School of Medicine, New York, NY, USA.

Yiqin Du (Y)

Department of Ophthalmology, University of Pittsburgh, Pittsburgh, PA, USA.
McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
Department of Developmental Biology, University of Pittsburgh, Pittsburgh, PA, USA.

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