Expanded sequential quadriplex real-time polymerase chain reaction (PCR) for identifying pneumococcal serotypes, penicillin susceptibility, and resistance markers.

Anti-Bacterial Agents / pharmacology Drug Resistance, Bacterial / genetics Humans Microbial Sensitivity Tests Multiplex Polymerase Chain Reaction / methods Penicillins / pharmacology Serogroup Streptococcus pneumoniae / drug effects
Antibiotic resistance Multiplex real-time PCR S. pneumoniae Serotyping

Journal

Diagnostic microbiology and infectious disease
ISSN: 1879-0070
Titre abrégé: Diagn Microbiol Infect Dis
Pays: United States
ID NLM: 8305899

Informations de publication

Date de publication:
Jun 2020
Historique:
received: 10 01 2020
revised: 09 03 2020
accepted: 09 03 2020
pubmed: 9 4 2020
medline: 28 11 2020
entrez: 9 4 2020
Statut: ppublish

Résumé

We expanded our current Centers for Disease Control and Prevention triplexed real-time polymerase chain reaction scheme identifying 11 individual serotypes and 10 serogroups to a quadriplex format identifying 34 individual serotypes and 13 small serogroups, 4 antibiotic resistance determinants, pilus targets, and penicillin susceptibility. Newly developed assays are specific for serotypes/serogroups, are sensitive (10 copies/reaction), and further discriminate larger serogroups into individual serotypes or smaller serogroups.

Identifiants

DOI: 10.1016/j.diagmicrobio.2020.115037 PMID: 32265073
pubmed: 32265073
pii: S0732-8893(20)30017-1
doi: 10.1016/j.diagmicrobio.2020.115037
pii:
doi:

Substances chimiques

Anti-Bacterial Agents 0
Penicillins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

115037

Informations de copyright

Copyright © 2020 Elsevier Inc. All rights reserved.

Auteurs

Srinivasan Velusamy (S)

Respiratory Diseases Branch, Division of Bacterial Disease, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA. Electronic address: SVelusamy@cdc.gov.

Theresa Tran (T)

IHRC, Atlanta, GA, USA.

Thitipong Mongkolrattanothai (T)

Rollins School of Public Health, Emory University, Atlanta, GA, USA.

Hollis Walker (H)

Respiratory Diseases Branch, Division of Bacterial Disease, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.

Lesley McGee (L)

Respiratory Diseases Branch, Division of Bacterial Disease, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.

Bernard Beall (B)

Respiratory Diseases Branch, Division of Bacterial Disease, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.

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Classifications MeSH

questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Utilisations thérapeutiques › Anti-infectieux › Antibactériens › Expanded sequential quadriplex real-time...
questionsmedicales.fr › Phénomènes physiologiques › Phénomènes pharmacologiques et toxicologiques › Phénomènes pharmacologiques › Résistance aux substances › Résistance microbienne aux médicaments › Résistance bactérienne aux médicaments › Expanded sequential quadriplex real-time...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Expanded sequential quadriplex real-time...
questionsmedicales.fr › Techniques d'investigation › Évaluation préclinique de médicament › Tests de sensibilité microbienne › Expanded sequential quadriplex real-time...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Réaction de polymérisation en chaine multiplex › Expanded sequential quadriplex real-time...
questionsmedicales.fr › Composés hétérocycliques › Composés hétérocycliques à cycles fusionnés › Composés hétérobicycliques › bêta-Lactames › Pénicillines › Expanded sequential quadriplex real-time...
questionsmedicales.fr › Phénomènes génétiques › Phénotype › Sérogroupe › Expanded sequential quadriplex real-time...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus pneumoniae › Expanded sequential quadriplex real-time...