Optical flow analysis reveals that Kinesin-mediated advection impacts the orientation of microtubules in the
Actins
/ metabolism
Animals
Biomechanical Phenomena
Cell Polarity
Cytoplasm
/ metabolism
Cytoplasmic Streaming
/ physiology
Cytoskeleton
/ metabolism
Drosophila Proteins
/ metabolism
Drosophila melanogaster
/ metabolism
Dyneins
/ metabolism
Female
Kinesins
/ metabolism
Mechanical Phenomena
Microtubules
/ metabolism
Oocytes
/ metabolism
Optic Flow
Orientation, Spatial
/ physiology
Journal
Molecular biology of the cell
ISSN: 1939-4586
Titre abrégé: Mol Biol Cell
Pays: United States
ID NLM: 9201390
Informations de publication
Date de publication:
01 06 2020
01 06 2020
Historique:
pubmed:
9
4
2020
medline:
29
5
2021
entrez:
9
4
2020
Statut:
ppublish
Résumé
The orientation of microtubule (MT) networks is exploited by motors to deliver cargoes to specific intracellular destinations and is thus essential for cell polarity and function. Reconstituted in vitro systems have largely contributed to understanding the molecular framework regulating the behavior of MT filaments. In cells, however, MTs are exposed to various biomechanical forces that might impact on their orientation, but little is known about it. Oocytes, which display forceful cytoplasmic streaming, are excellent model systems to study the impact of motion forces on cytoskeletons in vivo. Here we implement variational optical flow analysis as a new approach to analyze the polarity of MTs in the
Identifiants
pubmed: 32267197
doi: 10.1091/mbc.E19-08-0440
pmc: PMC7353148
doi:
Substances chimiques
Actins
0
Drosophila Proteins
0
Dyneins
EC 3.6.4.2
Kinesins
EC 3.6.4.4
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1246-1258Références
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