OZF is a Claspin-interacting protein essential to maintain the replication fork progression rate under replication stress.
Adaptor Proteins, Signal Transducing
/ chemistry
Carrier Proteins
/ chemistry
Cell Cycle
Cell Line
DNA Replication
/ physiology
HCT116 Cells
HEK293 Cells
HeLa Cells
Humans
Kruppel-Like Transcription Factors
/ chemistry
Protein Binding
Protein Interaction Domains and Motifs
RNA Interference
RNA-Binding Proteins
/ chemistry
Recombinant Proteins
/ chemistry
Stress, Physiological
Two-Hybrid System Techniques
Claspin
IPOND
OZF
ZNF146
replication stress
replisome
zinc-finger protein
Journal
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
ISSN: 1530-6860
Titre abrégé: FASEB J
Pays: United States
ID NLM: 8804484
Informations de publication
Date de publication:
05 2020
05 2020
Historique:
received:
30
07
2019
revised:
10
03
2020
accepted:
16
03
2020
pubmed:
9
4
2020
medline:
28
1
2021
entrez:
9
4
2020
Statut:
ppublish
Résumé
DNA replication is essential for cell proliferation and is one of the cell cycle stages where DNA is more vulnerable. Replication stress is a prominent property of tumor cells and an emerging target for cancer therapy. Although it is not directly involved in nucleotide incorporation, Claspin is a protein with relevant functions in DNA replication. It harbors a DNA-binding domain that interacts preferentially with branched or forked DNA molecules. It also acts as a platform for the interaction of proteins related to DNA damage checkpoint activation, DNA repair, DNA replication origin firing, and fork progression. In order to find new proteins potentially involved in the regulation of DNA replication, we performed a two-hybrid screen to discover new Claspin-binding proteins. This system allowed us to identify the zinc-finger protein OZF (ZNF146) as a new Claspin-interacting protein. OZF is also present at replication forks and co-immunoprecipitates not only with Claspin but also with other replisome components. Interestingly, OZF depletion does not affect DNA replication in a normal cell cycle, but its depletion induces a reduction in the fork progression rate under replication stress conditions. Our results suggest that OZF is a Claspin-binding protein with a specific function in fork progression under replication stress.
Identifiants
pubmed: 32267586
doi: 10.1096/fj.201901926R
doi:
Substances chimiques
Adaptor Proteins, Signal Transducing
0
CLSPN protein, human
0
Carrier Proteins
0
Kruppel-Like Transcription Factors
0
RNA-Binding Proteins
0
Recombinant Proteins
0
ZNF146 protein, human
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6907-6919Informations de copyright
© 2020 Federation of American Societies for Experimental Biology.
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