A Modular Differentiation System Maps Multiple Human Kidney Lineages from Pluripotent Stem Cells.
collecting duct
differentiation
induced pluripotent stem cells
kidney
mesonephros
metanephros
nephrogenesis
organoid
single-cell analysis
ureteric bud
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
07 04 2020
07 04 2020
Historique:
received:
26
03
2018
revised:
17
01
2020
accepted:
13
03
2020
entrez:
9
4
2020
pubmed:
9
4
2020
medline:
28
4
2021
Statut:
ppublish
Résumé
Recent studies using human pluripotent stem cells (hPSCs) have developed protocols to induce kidney-lineage cells and reconstruct kidney organoids. However, the separate generation of metanephric nephron progenitors (NPs), mesonephric NPs, and ureteric bud (UB) cells, which constitute embryonic kidneys, in in vitro differentiation culture systems has not been fully investigated. Here, we create a culture system in which these mesoderm-like cell types and paraxial and lateral plate mesoderm-like cells are separately generated from hPSCs. We recapitulate nephrogenic niches from separately induced metanephric NP-like and UB-like cells, which are subsequently differentiated into glomeruli, renal tubules, and collecting ducts in vitro and further vascularized in vivo. Our selective differentiation protocols should contribute to understanding the mechanisms underlying human kidney development and disease and also supply cell sources for regenerative therapies.
Identifiants
pubmed: 32268094
pii: S2211-1247(20)30354-5
doi: 10.1016/j.celrep.2020.03.040
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
107476Informations de copyright
Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests K.O. is a founder and member without salary of the scientific advisory boards of iPS Portal and a founder and the chief scientific advisor of RegeNephro. T.A. is a founder and scientific advisor of RegeNephro Co., Ltd. Differentiation method for nephron progenitor cells from hiPSCs (PCT/JP2018/019886, WO 2018/216743).