Gold Nanoparticles Mediate Improved Detection of β-amyloid Aggregates by Fluorescence.
Alzheimer´s disease
CRANAD-2
SEF
amyloid beta peptide
gold nanorods
Journal
Nanomaterials (Basel, Switzerland)
ISSN: 2079-4991
Titre abrégé: Nanomaterials (Basel)
Pays: Switzerland
ID NLM: 101610216
Informations de publication
Date de publication:
06 Apr 2020
06 Apr 2020
Historique:
received:
11
03
2020
revised:
28
03
2020
accepted:
29
03
2020
entrez:
10
4
2020
pubmed:
10
4
2020
medline:
10
4
2020
Statut:
epublish
Résumé
The early detection of the amyloid beta peptide aggregates involved in Alzheimer's disease is crucial to test new potential treatments. In this research, we improved the detection of amyloid beta peptide aggregates in vitro and ex vivo by fluorescence combining the use of CRANAD-2 and gold nanorods (GNRs) by the surface enhancement fluorescence effect. We synthetized GNRs and modified their surface with HS-PEG-OMe and HS-PEG-COOH and functionalized them with the D1 peptide, which has the capability to selectively bind to amyloid beta peptide. For an in vitro detection of amyloid beta peptide, we co-incubated amyloid beta peptide aggregates with the probe CRANAD-2 and GNR-PEG-D1 observing an increase in the intensity of the fluorescence signal attributed to surface enhancement fluorescence. Furthermore, the surface enhancement fluorescence effect was observed in brain slices of transgenic mice with Alzheimer´s disease co-incubated with CRANAD-2 and GNR-PEG-D1. An increase in the fluorescence signal was observed allowing the detection of aggregates that cannot be detected with the single use of CRANAD-2. Gold nanoparticles allowed an improvement in the detection of the amyloid aggregated by fluorescence in vitro and ex vivo.
Identifiants
pubmed: 32268543
pii: nano10040690
doi: 10.3390/nano10040690
pmc: PMC7221977
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Fondap
ID : 1513001
Organisme : Fondecyt
ID : 1170929
Organisme : Fondecyt
ID : 1161775
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