Development and production of nanobodies specifically against green fluorescence protein.
GFP nanobody
Phage display
VHH
Journal
Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612
Informations de publication
Date de publication:
Jun 2020
Jun 2020
Historique:
received:
02
12
2019
accepted:
09
03
2020
revised:
28
02
2020
pubmed:
10
4
2020
medline:
2
2
2021
entrez:
10
4
2020
Statut:
ppublish
Résumé
Variable domains of heavy chains of camelid heavy-chain antibodies (VHHs) are known as nanobodies. Nanobodies are approximately 15 kDa in size with high affinity to their antigens. They can be easily manipulated and produced in microorganisms. In this study, an alpaca was immunized with purified green fluorescence protein (GFP) and a VHH library from lymphocytes of the immunized alpaca was constructed with a capacity of 6.7 × 10
Identifiants
pubmed: 32270250
doi: 10.1007/s00253-020-10535-w
pii: 10.1007/s00253-020-10535-w
doi:
Substances chimiques
Peptide Library
0
Single-Domain Antibodies
0
Green Fluorescent Proteins
147336-22-9
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
4837-4848Subventions
Organisme : Fund from Guangzhou Institute of Pediatrics, Guangzhou Women and Children's Medical Center
ID : YIP-2019-031
Organisme : Research Start-up Fund for Guangdong Second Provincial General Hospital Introduced Talent
ID : YY2018-006