Cassava shrunken-2 homolog MeAPL3 determines storage root starch and dry matter content and modulates storage root postharvest physiological deterioration.
ADP-glucose pyrophophorylase
Cassava
Dry matter
MeAPL3
Postharvest physiological deterioration
Starch
Journal
Plant molecular biology
ISSN: 1573-5028
Titre abrégé: Plant Mol Biol
Pays: Netherlands
ID NLM: 9106343
Informations de publication
Date de publication:
Jun 2022
Jun 2022
Historique:
received:
19
12
2019
accepted:
05
03
2020
pubmed:
10
4
2020
medline:
7
6
2022
entrez:
10
4
2020
Statut:
ppublish
Résumé
Among the five cassava isoforms (MeAPL1-MeAPL5), MeAPL3 is responsible for determining storage root starch content. Degree of storage root postharvest physiological deterioration (PPD) is directly correlated with starch content. AGPase is heterotetramer composed of two small and two large subunits each coded by small gene families in higher plants. Studies in cassava (Manihot esculenta) identified and characterized five isoforms of Manihot esculenta ADP-glucose pyrophosphorylase large subunit (MeAPL1-MeAPL5) and employed virus induced gene silencing (VIGS) to show that MeAPL3 is the key isoform responsible for starch and dry matter accumulation in cassava storage roots. Silencing of MeAPL3 in cassava through stable transgenic lines resulted in plants displaying significant reduction in storage root starch and dry matter content (DMC) and induced a distinct phenotype associated with increased petiole/stem angle, resulting in a droopy leaf phenotype. Plants with reduced starch and DMC also displayed significantly reduced or no postharvest physiological deterioration (PPD) compared to controls and lines with high DMC and starch content. This provides strong evidence for direct relationships between starch/dry matter content and its role in PPD and canopy architecture traits in cassava.
Identifiants
pubmed: 32270429
doi: 10.1007/s11103-020-00995-z
pii: 10.1007/s11103-020-00995-z
pmc: PMC9163024
doi:
Substances chimiques
Starch
9005-25-8
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
283-299Subventions
Organisme : Bill and Melinda Gates Foundation
ID : OPPGD1484
Informations de copyright
© 2020. The Author(s).
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