Regulated lipid synthesis and LEM2/CHMP7 jointly control nuclear envelope closure.
Animals
Chromatin
/ genetics
Endoplasmic Reticulum
/ genetics
Endosomal Sorting Complexes Required for Transport
/ genetics
HeLa Cells
Humans
Lipids
/ biosynthesis
Membrane Proteins
/ genetics
Mice
Microtubules
/ genetics
Mitosis
/ genetics
Nuclear Envelope
/ genetics
Nuclear Proteins
/ genetics
Organic Chemicals
Phosphoprotein Phosphatases
/ genetics
Spindle Apparatus
/ genetics
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
04 05 2020
04 05 2020
Historique:
received:
24
08
2019
revised:
22
01
2020
accepted:
03
03
2020
entrez:
10
4
2020
pubmed:
10
4
2020
medline:
17
2
2021
Statut:
ppublish
Résumé
The nuclear permeability barrier depends on closure of nuclear envelope (NE) holes. Here, we investigate closure of the NE opening surrounding the meiotic spindle in C. elegans oocytes. ESCRT-III components accumulate at the opening but are not required for nuclear closure on their own. 3D analysis revealed cytoplasmic membranes directly adjacent to NE holes containing meiotic spindle microtubules. We demonstrate that the NE protein phosphatase, CNEP-1/CTDNEP1, controls de novo glycerolipid synthesis through lipin to prevent invasion of excess ER membranes into NE holes and a defective NE permeability barrier. Loss of NE adaptors for ESCRT-III exacerbates ER invasion and nuclear permeability defects in cnep-1 mutants, suggesting that ESCRTs restrict excess ER membranes during NE closure. Restoring glycerolipid synthesis in embryos deleted for CNEP-1 and ESCRT components rescued NE permeability defects. Thus, regulating the production and feeding of ER membranes into NE holes together with ESCRT-mediated remodeling is required for nuclear closure.
Identifiants
pubmed: 32271860
pii: 151636
doi: 10.1083/jcb.201908179
pmc: PMC7199858
pii:
doi:
Substances chimiques
CHMP7 protein, human
0
Chromatin
0
Endosomal Sorting Complexes Required for Transport
0
LEMD2 protein, human
0
Lipids
0
Membrane Proteins
0
Nuclear Proteins
0
Organic Chemicals
0
lipine
0
CTDNEP1 protein, human
EC 3.1.3.16
Phosphoprotein Phosphatases
EC 3.1.3.16
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM088151
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM134865
Pays : United States
Informations de copyright
© 2020 Penfield et al.
Références
Science. 2016 Apr 15;352(6283):353-8
pubmed: 27013428
Chromosome Res. 1993 May;1(1):15-26
pubmed: 8143084
J Cell Biol. 2019 Jul 1;218(7):2136-2149
pubmed: 31147383
Nucleus. 2012 Jan-Feb;3(1):88-100
pubmed: 22567193
J Biol Chem. 2008 Oct 24;283(43):29166-74
pubmed: 18694939
Curr Biol. 2018 Sep 24;28(18):2991-2997.e2
pubmed: 30197085
J Cell Sci. 2009 Jun 15;122(Pt 12):1970-8
pubmed: 19494126
J Biol Chem. 2006 Nov 10;281(45):34537-48
pubmed: 16968695
J Struct Biol. 1996 Jan-Feb;116(1):71-6
pubmed: 8742726
Proc Natl Acad Sci U S A. 2007 Apr 17;104(16):6596-601
pubmed: 17420445
Methods Cell Biol. 1995;48:303-21
pubmed: 8531731
Cold Spring Harb Perspect Biol. 2010 Oct;2(10):a000562
pubmed: 20630994
Genetics. 2015 Sep;201(1):47-54
pubmed: 26187122
WormBook. 2006 Jan 19;:1-40
pubmed: 18050484
EMBO J. 2005 Jun 1;24(11):1931-41
pubmed: 15889145
Curr Biol. 2016 Oct 10;26(19):2635-2641
pubmed: 27618263
Biochim Biophys Acta. 2013 Mar;1831(3):575-81
pubmed: 23026159
EMBO J. 2016 Nov 15;35(22):2447-2467
pubmed: 27733427
J Cell Sci. 2009 Jun 15;122(Pt 12):1963-9
pubmed: 19494125
Nat Rev Mol Cell Biol. 2008 Feb;9(2):112-24
pubmed: 18216768
Mol Biol Cell. 2018 Apr 1;29(7):852-868
pubmed: 29386297
Nature. 2015 Jun 11;522(7555):236-9
pubmed: 26040713
Cold Spring Harb Perspect Biol. 2010 Mar;2(3):a000539
pubmed: 20300205
J Biol Chem. 2012 Jan 27;287(5):3123-37
pubmed: 22134922
Nature. 2010 Apr 8;464(7290):864-9
pubmed: 20305637
Methods Cell Biol. 2007;79:101-19
pubmed: 17327153
Dev Cell. 2007 Apr;12(4):515-29
pubmed: 17419991
Dev Cell. 2018 Dec 3;47(5):547-563.e6
pubmed: 30513301
Science. 2016 Apr 15;352(6283):359-62
pubmed: 27013426
J Biol Chem. 2006 Apr 7;281(14):9210-8
pubmed: 16467296
Cell. 2016 Jul 28;166(3):664-678
pubmed: 27397507
Dev Biol. 2003 Aug 1;260(1):245-59
pubmed: 12885567
Curr Biol. 2016 Jan 25;26(2):237-243
pubmed: 26774782
Genetics. 2014 Nov;198(3):837-46
pubmed: 25161212
Cell. 2011 Aug 5;146(3):408-20
pubmed: 21816276
Microsc Microanal. 2014 Feb;20(1):152-63
pubmed: 24252586
Genes Dev. 2014 Jan 15;28(2):121-6
pubmed: 24449268
Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):E2166-E2175
pubmed: 28242692
Annu Rev Cell Dev Biol. 2018 Oct 6;34:85-109
pubmed: 30095293
Int Rev Cytol. 2001;205:149-214
pubmed: 11336391
Nucleus. 2015;6(2):102-6
pubmed: 25671705
Proc R Soc Lond B Biol Sci. 1972 Jul 25;182(1066):89-102
pubmed: 4403086
Curr Opin Cell Biol. 2011 Feb;23(1):78-84
pubmed: 20708397
J Cell Sci. 2002 Nov 15;115(Pt 22):4375-85
pubmed: 12376568
Methods Cell Biol. 2012;111:223-34
pubmed: 22857931
J Lipid Res. 2009 Apr;50 Suppl:S311-6
pubmed: 18952570
Nat Rev Mol Cell Biol. 2017 Apr;18(4):229-245
pubmed: 28120913
Nature. 2015 Jun 11;522(7555):231-5
pubmed: 26040712
Hum Mol Genet. 2011 Nov 1;20(21):4175-86
pubmed: 21831885
J Struct Biol. 1997 Dec;120(3):343-52
pubmed: 9441937