ARID1A ATR inhibition CRISPR/Cas9 HDAC inhibition SWI/SNF-complex germ cell tumors mass spectrometry molecular therapy

Journal

Cancers
ISSN: 2072-6694
Titre abrégé: Cancers (Basel)
Pays: Switzerland
ID NLM: 101526829

Informations de publication

Date de publication:
07 Apr 2020
Historique:
received: 07 03 2020
revised: 25 03 2020
accepted: 06 04 2020
entrez: 11 4 2020
pubmed: 11 4 2020
medline: 11 4 2020
Statut: epublish

Résumé

Germ cell tumors (GCTs) are the most common solid malignancies found in young men. Although they generally have high cure rates, metastases, resistance to cisplatin-based therapy, and late toxicities still represent a lethal threat, arguing for the need of new therapeutic options. In a previous study, we identified downregulation of the chromatin-remodeling SWI/SNF complex member ARID1A as a key event in the mode of action of the histone deacetylase inhibitor romidepsin. Additionally, the loss-of-function mutations re-sensitize different tumor types to various drugs, like EZH2-, PARP-, HDAC-, HSP90- or ATR-inhibitors. Thus, ARID1A presents as a promising target for synthetic lethality and combination therapy. In this study, we deciphered the molecular function of ARID1A and screened for the potential of two pharmacological ARID1A inhibitors as a new therapeutic strategy to treat GCTs. By CRISPR/Cas9, we generated

Identifiants

pubmed: 32272809
pii: cancers12040905
doi: 10.3390/cancers12040905
pmc: PMC7226530
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : Düsseldorf School of Oncology (DSO)
ID : Funding of BMFZ Mass Spectrometry

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Auteurs

Lukas Kurz (L)

Department of Urology, Urological Research Lab, Translational UroOncology, University Hospital Düsseldorf, 40225 Düsseldorf, Germany.

Alissa Miklyaeva (A)

Department of Urology, Urological Research Lab, Translational UroOncology, University Hospital Düsseldorf, 40225 Düsseldorf, Germany.

Margaretha A Skowron (MA)

Department of Urology, Urological Research Lab, Translational UroOncology, University Hospital Düsseldorf, 40225 Düsseldorf, Germany.

Nina Overbeck (N)

Molecular Proteomics Laboratory, Heinrich-Heine-University Düsseldorf, 40225 Düsseldorf, Germany.
Institute for Molecular Medicine I, Medical Faculty, Heinrich-Heine-University Düsseldorf, 40225 Düsseldorf, Germany.

Gereon Poschmann (G)

Molecular Proteomics Laboratory, Heinrich-Heine-University Düsseldorf, 40225 Düsseldorf, Germany.
Institute for Molecular Medicine I, Medical Faculty, Heinrich-Heine-University Düsseldorf, 40225 Düsseldorf, Germany.

Teresa Becker (T)

Department of Urology, Urological Research Lab, Translational UroOncology, University Hospital Düsseldorf, 40225 Düsseldorf, Germany.

Katharina Eul (K)

Department of Urology, Urological Research Lab, Translational UroOncology, University Hospital Düsseldorf, 40225 Düsseldorf, Germany.

Thomas Kurz (T)

Institute for Pharmaceutical and Medicinal Chemistry, Heinrich Heine University Düsseldorf, 40225 Düsseldorf, Germany.

Stefan Schönberger (S)

Department of Paediatric Haematology and Oncology, University Hospital Bonn, 53113 Bonn, Germany.

Gabriele Calaminus (G)

Department of Paediatric Haematology and Oncology, University Hospital Bonn, 53113 Bonn, Germany.

Kai Stühler (K)

Institute for Molecular Medicine I, Medical Faculty, Heinrich-Heine-University Düsseldorf, 40225 Düsseldorf, Germany.

Emily Dykhuizen (E)

Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 479078, USA.

Peter Albers (P)

Department of Urology, University Hospital Düsseldorf, 40225 Düsseldorf, Germany.

Daniel Nettersheim (D)

Department of Urology, Urological Research Lab, Translational UroOncology, University Hospital Düsseldorf, 40225 Düsseldorf, Germany.

Classifications MeSH