Intravital imaging of mouse embryos.
Animals
Brain
/ embryology
Cell Division
Cell Movement
Chimera
/ embryology
Electroporation
Embryo, Mammalian
/ cytology
Embryonic Development
Female
Gene Transfer Techniques
Intravital Microscopy
/ methods
Mice
Mice, Transgenic
Neovascularization, Physiologic
Neural Crest
/ blood supply
Placenta
/ physiology
Pregnancy
Retina
/ embryology
Synaptic Transmission
Uterus
Journal
Science (New York, N.Y.)
ISSN: 1095-9203
Titre abrégé: Science
Pays: United States
ID NLM: 0404511
Informations de publication
Date de publication:
10 04 2020
10 04 2020
Historique:
received:
29
10
2019
accepted:
16
03
2020
entrez:
11
4
2020
pubmed:
11
4
2020
medline:
18
4
2020
Statut:
ppublish
Résumé
Embryonic development is a complex process that is unamenable to direct observation. In this study, we implanted a window to the mouse uterus to visualize the developing embryo from embryonic day 9.5 to birth. This removable intravital window allowed manipulation and high-resolution imaging. In live mouse embryos, we observed transient neurotransmission and early vascularization of neural crest cell (NCC)-derived perivascular cells in the brain, autophagy in the retina, viral gene delivery, and chemical diffusion through the placenta. We combined the imaging window with in utero electroporation to label and track cell division and movement within embryos and observed that clusters of mouse NCC-derived cells expanded in interspecies chimeras, whereas adjacent human donor NCC-derived cells shrank. This technique can be combined with various tissue manipulation and microscopy methods to study the processes of development at unprecedented spatiotemporal resolution.
Identifiants
pubmed: 32273467
pii: 368/6487/181
doi: 10.1126/science.aba0210
pmc: PMC7646360
mid: NIHMS1638993
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
181-186Subventions
Organisme : NICHD NIH HHS
ID : R01 HD039963
Pays : United States
Organisme : NINDS NIH HHS
ID : R21 NS098176
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH104610
Pays : United States
Organisme : NCI NIH HHS
ID : R35 CA197616
Pays : United States
Organisme : NICHD NIH HHS
ID : R37 HD045022
Pays : United States
Organisme : NIMH NIH HHS
ID : R21 MH119813
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS110388
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM122465
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS083897
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS088538
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK119795
Pays : United States
Organisme : NCI NIH HHS
ID : U01 CA214300
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
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