An intracellular membrane protein GEP1 regulates xanthurenic acid induced gametogenesis of malaria parasites.
Animals
CRISPR-Cas Systems
/ genetics
Calcium
/ metabolism
Culicidae
/ metabolism
Cyclic GMP
/ metabolism
Cyclic GMP-Dependent Protein Kinases
/ metabolism
Gametogenesis
/ drug effects
Gene Editing
/ methods
Intracellular Membranes
/ metabolism
Malaria
/ parasitology
Mosquito Vectors
/ metabolism
Plasmodium
/ genetics
Protozoan Proteins
/ genetics
Xanthurenates
/ metabolism
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
09 04 2020
09 04 2020
Historique:
received:
01
08
2019
accepted:
14
03
2020
entrez:
11
4
2020
pubmed:
11
4
2020
medline:
28
7
2020
Statut:
epublish
Résumé
Gametocytes differentiation to gametes (gametogenesis) within mosquitos is essential for malaria parasite transmission. Both reduction in temperature and mosquito-derived XA or elevated pH are required for triggering cGMP/PKG dependent gametogenesis. However, the parasite molecule for sensing or transducing these environmental signals to initiate gametogenesis remains unknown. Here we perform a CRISPR/Cas9-based functional screening of 59 membrane proteins expressed in the gametocytes of Plasmodium yoelii and identify that GEP1 is required for XA-stimulated gametogenesis. GEP1 disruption abolishes XA-stimulated cGMP synthesis and the subsequent signaling and cellular events, such as Ca
Identifiants
pubmed: 32273496
doi: 10.1038/s41467-020-15479-3
pii: 10.1038/s41467-020-15479-3
pmc: PMC7145802
doi:
Substances chimiques
Protozoan Proteins
0
Xanthurenates
0
xanthurenic acid
58LAB1BG8J
Cyclic GMP-Dependent Protein Kinases
EC 2.7.11.12
Cyclic GMP
H2D2X058MU
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1764Références
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