Poly(N-isopropylacrylamide) based thin microgel films for use in cell culture applications.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
09 04 2020
09 04 2020
Historique:
received:
14
12
2019
accepted:
20
03
2020
entrez:
11
4
2020
pubmed:
11
4
2020
medline:
25
11
2020
Statut:
epublish
Résumé
Poly(N-isopropylacrylamide) (PNIPAm) is widely used to fabricate cell sheet surfaces for cell culturing, however copolymer and interpenetrated polymer networks based on PNIPAm have been rarely explored in the context of tissue engineering. Many complex and expensive techniques have been employed to produce PNIPAm-based films for cell culturing. Among them, spin coating has demonstrated to be a rapid fabrication process of thin layers with high reproducibility and uniformity. In this study, we introduce an innovative approach to produce anchored smart thin films both thermo- and electro-responsive, with the aim to integrate them in electronic devices and better control or mimic different environments for cells in vitro. Thin films were obtained by spin coating of colloidal solutions made by PNIPAm and PAAc nanogels. Anchoring the films to the substrates was obtained through heat treatment in the presence of dithiol molecules. From analyses carried out with AFM and XPS, the final samples exhibited a flat morphology and high stability to water washing. Viability tests with cells were finally carried out to demonstrate that this approach may represent a promising route to integrate those hydrogels films in electronic platforms for cell culture applications.
Identifiants
pubmed: 32273560
doi: 10.1038/s41598-020-63228-9
pii: 10.1038/s41598-020-63228-9
pmc: PMC7145875
doi:
Substances chimiques
Acrylic Resins
0
Hydrogels
0
poly-N-isopropylacrylamide
25189-55-3
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6126Références
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