Establishing a pre-clinical growing animal model to test a tissue engineered valved pulmonary conduit.

CorMATRIX Tissue engineering animal model pulmonary valve pulmonary valved conduit

Journal

Journal of thoracic disease
ISSN: 2072-1439
Titre abrégé: J Thorac Dis
Pays: China
ID NLM: 101533916

Informations de publication

Date de publication:
Mar 2020
Historique:
entrez: 11 4 2020
pubmed: 11 4 2020
medline: 11 4 2020
Statut: ppublish

Résumé

Many valvular pathologies of the heart may be only sufficiently treated by replacement of the valve if a reconstruction is not feasible. However, structural deterioration, thrombosis with thromboembolic events and infective endocarditis are commonly encountered complications over time and often demand a re-operation. In congenital heart disease the lack of small diameter valves with the potential to grow poses additional challenges and limits treatment options to homo- or xenograft implants. In this study, a chronic sheep model (24 months follow-up), a self-constructed valved conduit was created out of a tissue engineered (TE) patch (CorMatrix® Cardiovascular, Inc, USA) and implanted in orthotopic right ventricular (RV)-pulmonary artery (PA) position. Thereafter, the sheep were regularly monitored by clinical, laboratory and echocardiographic examinations to evaluate cardiac function and the implanted RV-PA-conduit. Here, we summarize the study protocol and our experiences during the perioperative phase and the follow up period and explain how we constructed a valved conduit out of a commercially available TE patch. License number: ZH 284/14.

Sections du résumé

BACKGROUND BACKGROUND
Many valvular pathologies of the heart may be only sufficiently treated by replacement of the valve if a reconstruction is not feasible. However, structural deterioration, thrombosis with thromboembolic events and infective endocarditis are commonly encountered complications over time and often demand a re-operation. In congenital heart disease the lack of small diameter valves with the potential to grow poses additional challenges and limits treatment options to homo- or xenograft implants.
METHODS METHODS
In this study, a chronic sheep model (24 months follow-up), a self-constructed valved conduit was created out of a tissue engineered (TE) patch (CorMatrix® Cardiovascular, Inc, USA) and implanted in orthotopic right ventricular (RV)-pulmonary artery (PA) position. Thereafter, the sheep were regularly monitored by clinical, laboratory and echocardiographic examinations to evaluate cardiac function and the implanted RV-PA-conduit.
DISCUSSION CONCLUSIONS
Here, we summarize the study protocol and our experiences during the perioperative phase and the follow up period and explain how we constructed a valved conduit out of a commercially available TE patch.
TRIAL REGISTRATION BACKGROUND
License number: ZH 284/14.

Identifiants

pubmed: 32274175
doi: 10.21037/jtd.2019.12.70
pii: jtd-12-03-1070
pmc: PMC7138975
doi:

Types de publication

Journal Article

Langues

eng

Pagination

1070-1078

Informations de copyright

2020 Journal of Thoracic Disease. All rights reserved.

Déclaration de conflit d'intérêts

Conflicts of Interest: The authors have no conflicts of interest to declare.

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Auteurs

Walter Knirsch (W)

Division of Pediatric Cardiology, Pediatric Heart Center, University Children's Hospital Zurich, Zurich, Switzerland.
Children's Research Center, University Children's Hospital Zurich, Zurich, Switzerland.

Bernard Krüger (B)

Division of Cardiac Anesthesia, Institute of Anesthesiology, University Hospital Zurich, Zurich, Switzerland.
Department of Anesthesia, University Children's Hospital, Zurich, Switzerland.

Thea Fleischmann (T)

Division of Surgical Research, University Hospital Zurich, Zurich, Switzerland.

Alexandra Malbon (A)

Institute for Veterinary Pathology Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.

Miriam Lipiski (M)

Division of Surgical Research, University Hospital Zurich, Zurich, Switzerland.

Frithjof Lemme (F)

Children's Research Center, University Children's Hospital Zurich, Zurich, Switzerland.
Department of Congenital Cardiovascular Surgery, Pediatric Heart Center, University Children's Hospital Zurich, Switzerland.

Mareike Sauer (M)

Division of Surgical Research, University Hospital Zurich, Zurich, Switzerland.

Niko Cesarovic (N)

Division of Surgical Research, University Hospital Zurich, Zurich, Switzerland.

Hitendu Dave (H)

Department of Congenital Cardiovascular Surgery, Pediatric Heart Center, University Children's Hospital Zurich, Switzerland.

Michael Hübler (M)

Children's Research Center, University Children's Hospital Zurich, Zurich, Switzerland.
Department of Congenital Cardiovascular Surgery, Pediatric Heart Center, University Children's Hospital Zurich, Switzerland.

Martin Schweiger (M)

Children's Research Center, University Children's Hospital Zurich, Zurich, Switzerland.
Department of Congenital Cardiovascular Surgery, Pediatric Heart Center, University Children's Hospital Zurich, Switzerland.

Classifications MeSH