Synchronous genitourinary lichen sclerosus signals a distinct urinary microbiome profile in men with urethral stricture disease.
Colonization
Lichen sclerosus
Microbiome
Urethral stricture disease
Urinary tract infection (UTI)
Journal
World journal of urology
ISSN: 1433-8726
Titre abrégé: World J Urol
Pays: Germany
ID NLM: 8307716
Informations de publication
Date de publication:
Feb 2021
Feb 2021
Historique:
received:
24
02
2020
accepted:
31
03
2020
pubmed:
11
4
2020
medline:
23
7
2021
entrez:
11
4
2020
Statut:
ppublish
Résumé
Alterations in the urinary microbiome have been associated with urological diseases. The microbiome of patients with urethral stricture disease (USD) remains unknown. Our objective is to examine the microbiome of USD with a focus on inflammatory USD caused by lichen sclerosus (LS). We collected mid-stream urine samples from men with LS-USD (cases; n = 22) and non-LS USD (controls; n = 76). DNA extraction, PCR amplification of the V4 hypervariable region of the 16S rRNA gene, and sequencing was done on the samples. Operational taxonomic units (OTUs) were defined using a > 97% sequence similarity threshold. Alpha diversity measurements of diversity, including microbiome richness (number of different OTUs) and evenness (distribution of OTUs) were calculated and compared. Microbiome beta diversity (difference between microbial communities) relationships with cases and controls were also assessed. Fifty specimens (13 cases and 37 controls) produced a 16S rRNA amplicon. Mean sample richness was 25.9 vs. 16.8 (p = 0.076) for LS-USD vs. non-LS USD, respectively. LS-USD had a unique profile of bacteria by taxonomic order including Bacillales, Bacteroidales and Pasteurellales enriched urine. The beta variation of observed bacterial communities was best explained by the richness. Men with LS-USD may have a unique microbiologic richness, specifically inclusive of Bacillales, Bacteroidales and Pasteurellales enriched urine compared to those with non-LS USD. Further work will be required to elucidate the clinical relevance of these variations in the urinary microbiome.
Identifiants
pubmed: 32274566
doi: 10.1007/s00345-020-03198-9
pii: 10.1007/s00345-020-03198-9
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
605-611Subventions
Organisme : Alafi Foundation
ID : 10001
Références
Drake MJ, Morris N, Apostolidis A et al (2017) The urinary microbiome and its contribution to lower urinary tract symptoms; ICI-RS 2015. Neurourol Urodyn 36:850–853. https://doi.org/10.1002/nau.23006
doi: 10.1002/nau.23006
pubmed: 28444712
Bajic P, Van Kuiken ME, Burge BK et al (2018) Male Bladder Microbiome Relates to Lower Urinary Tract Symptoms. European Urology Focus. https://doi.org/10.1016/j.euf.2018.08.001
doi: 10.1016/j.euf.2018.08.001
pubmed: 30143471
Mueller ER, Wolfe AJ, Brubaker L (2017) Female urinary microbiota. Curr Opin Urol 27(3):282–286
doi: 10.1097/MOU.0000000000000396
Schneeweiss J, Koch M, Umek W (2016) The human urinary microbiome and how it relates to urogynecology. Int Urogynecol J 27:1307–1312. https://doi.org/10.1007/s00192-016-2944-5
doi: 10.1007/s00192-016-2944-5
pubmed: 26811114
Frølund M, Wikström A, Lidbrink P et al (2018) The bacterial microbiota in first-void urine from men with and without idiopathic urethritis. PLoS ONE 13:e0201380. https://doi.org/10.1371/journal.pone.0201380
doi: 10.1371/journal.pone.0201380
pubmed: 30052651
pmcid: 6063444
Dong Q, Nelson DE, Toh E et al (2011) The microbial communities in male first catch urine are highly similar to those in paired urethral swab specimens. PLoS ONE 6:e19709. https://doi.org/10.1371/journal.pone.0019709
doi: 10.1371/journal.pone.0019709
pubmed: 21603636
pmcid: 3094389
Belkaid Y, Hand TW (2014) Role of the microbiota in immunity and inflammation. Cell 157:121–141. https://doi.org/10.1016/j.cell.2014.03.011
doi: 10.1016/j.cell.2014.03.011
pubmed: 4056765
pmcid: 4056765
Buford TW (2017) (Dis)Trust your gut: the gut microbiome in age-related inflammation, health, and disease. Microbiome 5:80. https://doi.org/10.1186/s40168-017-0296-0
doi: 10.1186/s40168-017-0296-0
pubmed: 28709450
pmcid: 5512975
Fischbach MA, Segre JA (2016) Signaling in host-associated microbial communities. Cell 164:1288–1300. https://doi.org/10.1016/j.cell.2016.02.037
doi: 10.1016/j.cell.2016.02.037
pubmed: 26967294
pmcid: 4801507
Grimes MD, Tesdahl BA, Schubbe M et al (2019) Histopathology of anterior urethral strictures: towards a better understanding of stricture pathophysiology. J Urol. https://doi.org/10.1097/JU.0000000000000340
doi: 10.1097/JU.0000000000000340
pubmed: 31188733
pmcid: 6879783
Pugliese JM, Morey AF, Peterson AC (2007) Lichen sclerosus: review of the literature and current recommendations for management. J Urol 178:2268–2276. https://doi.org/10.1016/j.juro.2007.08.024
doi: 10.1016/j.juro.2007.08.024
pubmed: 17936829
Osterberg EC, Murphy G, Harris CR et al (2017) Cost-effective strategies for the management and treatment of urethral stricture disease. Urol Clin N Am 44:11–17. https://doi.org/10.1016/j.ucl.2016.08.002
doi: 10.1016/j.ucl.2016.08.002
Levy A, Browne B, Fredrick A et al (2019) Insights into the pathophysiology of urethral stricture disease due to lichen sclerosus: comparison of pathological markers in lichen sclerosus induced strictures vs nonlichen sclerosus induced strictures. J Urol 201:1158–1163. https://doi.org/10.1097/JU.0000000000000155
doi: 10.1097/JU.0000000000000155
pubmed: 30835614
Fujimura KE, Sitarik AR, Havstad S et al (2016) Neonatal gut microbiota associates with childhood multisensitized atopy and T cell differentiation. Nat Med 22:1187–1191. https://doi.org/10.1038/nm.4176
doi: 10.1038/nm.4176
pubmed: 27618652
pmcid: 5053876
Whittaker RH (1972) Evolution and measurement of species diversity. Taxon 21:213. https://doi.org/10.2307/1218190
doi: 10.2307/1218190
Caporaso JG, Kuczynski J, Stombaugh J et al (2010) QIIME allows analysis of high-throughput community sequencing data. Nat Methods 7:335–336. https://doi.org/10.1038/nmeth.f.303
doi: 10.1038/nmeth.f.303
pubmed: 20383131
pmcid: 20383131
Love MI, Huber W, Anders S (2014) Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol. https://doi.org/10.1186/s13059-014-0550-8
doi: 10.1186/s13059-014-0550-8
pubmed: 25516281
pmcid: 4302049
Pearce MM, Hilt EE, Rosenfeld AB et al (2014) The female urinary microbiome: a comparison of women with and without urgency urinary incontinence. Blaser MJ, ed. MBio. https://doi.org/10.1128/mBio.01283-14
doi: 10.1128/mBio.01283-14
pubmed: 25006228
pmcid: 4161260
Wu P, Zhang G, Zhao J et al (2018) Profiling the urinary microbiota in male patients with bladder cancer in China. Front Cell Infect Microbiol 8:167. https://doi.org/10.3389/fcimb.2018.00167
doi: 10.3389/fcimb.2018.00167
pubmed: 29904624
pmcid: 5990618
Shrestha E, White JR, Yu S-H et al (2018) Profiling the urinary microbiome in men with positive versus negative biopsies for prostate cancer. J Urol 199:161–171. https://doi.org/10.1016/j.juro.2017.08.001
doi: 10.1016/j.juro.2017.08.001
pubmed: 28797714
Gérard P (2016) Gut microbiota and obesity. Cell Mol Life Sci 73:147–162. https://doi.org/10.1007/s00018-015-2061-5
doi: 10.1007/s00018-015-2061-5
pubmed: 26459447
Carlson BC, Hofer MD, Ballek N et al (2013) Protein markers of malignant potential in penile and vulvar lichen sclerosus. J Urol 190:399–406. https://doi.org/10.1016/j.juro.2013.01.102
doi: 10.1016/j.juro.2013.01.102
pubmed: 23399649
Sfanos KS, Yegnasubramanian S, Nelson WG et al (2018) The inflammatory microenvironment and microbiome in prostate cancer development. Nat Rev Urol 15:11–24. https://doi.org/10.1038/nrurol.2017.167
doi: 10.1038/nrurol.2017.167
pubmed: 29089606
Rajpoot M, Sharma AK, Sharma A et al (2018) Understanding the microbiome: emerging biomarkers for exploiting the microbiota for personalized medicine against cancer. Semin Cancer Biol 52:1–8. https://doi.org/10.1016/j.semcancer.2018.02.003
doi: 10.1016/j.semcancer.2018.02.003
pubmed: 29425888
Bunker CB, Shim TN (2015) Male genital lichen sclerosus. Indian J Dermatol 60(2):111–117
doi: 10.4103/0019-5154.152501
Shogan BD, Smith DP, Christley S et al (2014) Intestinal anastomotic injury alters spatially defined microbiome composition and function. Microbiome 2:35. https://doi.org/10.1186/2049-2618-2-35
doi: 10.1186/2049-2618-2-35
pubmed: 25250176
pmcid: 4171717
Kassiri B, Shrestha E, Kasprenski M et al (2019) A prospective study of the urinary and gastrointestinal microbiome in prepubertal males. Urology. https://doi.org/10.1016/j.urology.2019.05.031
doi: 10.1016/j.urology.2019.05.031
pubmed: 31195012
Sathiananthamoorthy S, Malone-Lee J, Gill K et al (2018) Reassessment of routine midstream culture in diagnosis of urinary tract infection. Munson E, ed. J Clin Microbiol. https://doi.org/10.1128/JCM.01452-18
doi: 10.1128/JCM.01452-18
Pollock J, Glendinning L, Wisedchanwet T et al (2018) The madness of microbiome: attempting to find consensus “best practice” for 16S microbiome studies. Liu S-J, ed. Appl Environ Microbiol. https://doi.org/10.1128/AEM.02627-17
doi: 10.1128/AEM.02627-17
pubmed: 29427429
pmcid: 5861821
Brooks JP, Edwards DJ, Harwich MD et al (2015) The truth about metagenomics: quantifying and counteracting bias in 16S rRNA studies. BMC Microbiol 15:66. https://doi.org/10.1186/s12866-015-0351-6
doi: 10.1186/s12866-015-0351-6
pubmed: 25880246
pmcid: 4433096
Dill-McFarland KA, Tang Z-Z, Kemis JH et al (2019) Close social relationships correlate with human gut microbiota composition. Sci Rep 9:703. https://doi.org/10.1038/s41598-018-37298-9
doi: 10.1038/s41598-018-37298-9
pubmed: 30679677
pmcid: 6345772
Lax S, Sangwan N, Smith D et al (2017) Bacterial colonization and succession in a newly opened hospital. Sci Transl Med. https://doi.org/10.1126/scitranslmed.aah6500
doi: 10.1126/scitranslmed.aah6500
pubmed: 28539477
pmcid: 5706123