In situ hybridization of feline leukemia virus in a primary neural B-cell lymphoma.
B-cell
feline
in situ hybridization
leukemia virus
lymphoma
Journal
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
ISSN: 1943-4936
Titre abrégé: J Vet Diagn Invest
Pays: United States
ID NLM: 9011490
Informations de publication
Date de publication:
May 2020
May 2020
Historique:
pubmed:
11
4
2020
medline:
6
10
2020
entrez:
11
4
2020
Statut:
ppublish
Résumé
An 8-y-old castrated male, outdoor European shorthair cat was presented with a history of hindlimb weakness and paralysis. Disease progression was continuous from the onset; deep algesia disappeared at the final stage. Radiography of the vertebral column was unremarkable; along with patient history and physical examination results, magnetic resonance imaging suggested inflammatory lesions in the spinal cord, although neoplasia could not be ruled out. Feline leukemia virus (FeLV) positivity was confirmed by a serum ELISA prior to euthanasia. Upon postmortem examination, hemorrhages were present in the spinal cord at the level of vertebrae T7-8. Histologic and immunohistochemical analysis revealed primary diffuse large B-cell lymphoma of the spinal cord with multifocal myelomalacia and hemorrhages. To determine the presence of a pathogen within the lesion, we developed a novel in situ hybridization protocol for FeLV (RNAscope). The reaction revealed large amounts of FeLV viral RNA in the tumor cells.
Identifiants
pubmed: 32274976
doi: 10.1177/1040638720915449
pmc: PMC7377617
doi:
Substances chimiques
RNA, Viral
0
Types de publication
Case Reports
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
454-457Références
Vet Pathol. 2012 Jul;49(4):658-68
pubmed: 21505197
J Clin Virol. 2016 Apr;77:21-8
pubmed: 26875099
Vet Comp Oncol. 2010 Mar;8(1):38-49
pubmed: 20230580
JFMS Open Rep. 2015 Jul 29;1(2):2055116915598336
pubmed: 28491380
J Mol Diagn. 2012 Jan;14(1):22-9
pubmed: 22166544
J Vet Med Sci. 2013;75(6):701-7
pubmed: 23337319
J Vet Med Sci. 2014 Jun;76(6):807-11
pubmed: 24521793
J Neuroendocrinol. 2019 Mar;31(3):e12653
pubmed: 30362285
Cancer Res. 1985 Sep;45(9 Suppl):4534s-4538s
pubmed: 2990682
J Vet Diagn Invest. 2019 Jan;31(1):113-117
pubmed: 30541408
J Feline Med Surg. 2010 Dec;12(12):929-35
pubmed: 21036089
J Virol Methods. 2005 Dec;130(1-2):124-32
pubmed: 16054243
Viruses. 2012 Oct 31;4(11):2684-710
pubmed: 23202500
Vet Pathol. 2015 Mar;52(2):250-9
pubmed: 24903757
Nature. 1964 May 9;202:566-7
pubmed: 14195053
J Virol. 2016 Mar 28;90(8):4093-4104
pubmed: 26865706