Radial alignment of microtubules through tubulin polymerization in an evaporating droplet.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
18
12
2019
accepted:
21
03
2020
entrez:
11
4
2020
pubmed:
11
4
2020
medline:
8
7
2020
Statut:
epublish
Résumé
We report the formation of spherulites from droplets of highly concentrated tubulin solution via nucleation and subsequent polymerization to microtubules (MTs) under water evaporation by heating. Radial alignment of MTs in the spherulites was confirmed by the optical properties of the spherulites observed using polarized optical microscopy and fluorescence microscopy. Temperature and concentration of tubulins were found as important parameters to control the spherulite pattern formation of MTs where evaporation plays a significant role. The alignment of MTs was regulated reversibly by temperature induced polymerization and depolymerization of tubulins. The formation of the MTs patterns was also confirmed at the molecular level from the small angle X-ray measurements. This work provides a simple method for obtaining radially aligned arrays of MTs.
Identifiants
pubmed: 32275729
doi: 10.1371/journal.pone.0231352
pii: PONE-D-19-33923
pmc: PMC7147791
doi:
Substances chimiques
Tubulin
0
Water
059QF0KO0R
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0231352Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Eur Phys J E Soft Matter. 2010 Sep;33(1):19-26
pubmed: 20853173
Protein Expr Purif. 2003 Nov;32(1):83-8
pubmed: 14680943
J Mol Biol. 1987 Sep 20;197(2):205-18
pubmed: 3681995
J Cell Biol. 1986 Jun;102(6):2023-32
pubmed: 3519619
Langmuir. 2012 Mar 6;28(9):4039-42
pubmed: 22339498
Proc Natl Acad Sci U S A. 2004 Oct 5;101(40):14420-4
pubmed: 15381766
Phys Rev Lett. 2015 Apr 3;114(13):138102
pubmed: 25884139
Chem Soc Rev. 2017 Sep 18;46(18):5570-5587
pubmed: 28329028
Proc Natl Acad Sci U S A. 2006 Jul 11;103(28):10654-9
pubmed: 16818889
Biophys J. 1998 Jan;74(1):645-54
pubmed: 9449366
Nature. 2012 Mar 21;483(7390):448-52
pubmed: 22437613
Langmuir. 2012 Mar 20;28(11):4984-8
pubmed: 22369657
Nano Lett. 2005 Apr;5(4):629-33
pubmed: 15826099
J Am Chem Soc. 2009 Dec 23;131(50):18089-95
pubmed: 19928896
Nature. 2010 Sep 2;467(7311):73-7
pubmed: 20811454
Phys Rev E Stat Phys Plasmas Fluids Relat Interdiscip Topics. 1994 Aug;50(2):1579-1588
pubmed: 9962129
PLoS One. 2008 Jan 23;3(1):e1476
pubmed: 18213384
Soft Matter. 2015 May 21;11(19):3869-74
pubmed: 25864798
J Biol Chem. 1990 Jan 25;265(3):1639-47
pubmed: 2295647
Proc Natl Acad Sci U S A. 2014 Dec 16;111(50):17715-22
pubmed: 25468969
Cell Motil Cytoskeleton. 1988;10(1-2):229-36
pubmed: 2972399
Nat Methods. 2005 Apr;2(4):299-303
pubmed: 16167385
Cytoskeleton (Hoboken). 2012 Oct;69(10):738-50
pubmed: 22786885
Phys Rev E Stat Phys Plasmas Fluids Relat Interdiscip Topics. 2000 Jul;62(1 Pt B):756-65
pubmed: 11088531
Langmuir. 2004 Apr 13;20(8):3456-63
pubmed: 15875882
Annu Rev Cell Biol. 1985;1:145-72
pubmed: 3916316
Nature. 1997 Sep 18;389(6648):305-8
pubmed: 9305848
Sci Rep. 2015 Mar 31;5:9581
pubmed: 25823414
Trends Biochem Sci. 2001 Oct;26(10):597-604
pubmed: 11590012
Phys Rev Lett. 2003 Apr 4;90(13):138102
pubmed: 12689327
Science. 2014 Sep 5;345(6201):1135-9
pubmed: 25190790
Nature. 2012 Nov 15;491(7424):431-4
pubmed: 23135402
Curr Biol. 2004 Aug 24;14(16):1462-7
pubmed: 15324662
J Phys Chem B. 2011 Aug 25;115(33):9948-54
pubmed: 21786810
J Phys Chem B. 2012 Sep 27;116(38):11656-61
pubmed: 22953757
Sci Rep. 2017 Jul 21;7(1):6166
pubmed: 28733669
Sci Rep. 2018 Jun 25;8(1):9580
pubmed: 29942029
Colloids Surf B Biointerfaces. 2018 Jan 1;161:103-110
pubmed: 29055238