Nitrogen acquisition from mineral-associated proteins by an ectomycorrhizal fungus.
Paxillus involutus
boreal forests
decomposition and N acquisition
ectomycorrhizal (ECM) fungi
iron oxides
mineral-associated organic nitrogen
secondary metabolites
soil proteins
Journal
The New phytologist
ISSN: 1469-8137
Titre abrégé: New Phytol
Pays: England
ID NLM: 9882884
Informations de publication
Date de publication:
10 2020
10 2020
Historique:
received:
20
02
2020
accepted:
29
03
2020
pubmed:
13
4
2020
medline:
15
5
2021
entrez:
13
4
2020
Statut:
ppublish
Résumé
In nitrogen (N)-limited boreal forests, trees depend on the decomposing activity of their ectomycorrhizal (ECM) fungal symbionts to access soil N. A large fraction of this N exists as proteinaceous compounds associated with mineral particles. However, it is not known if ECM fungi can access these mineral-associated proteins; accordingly, possible acquisition mechanisms have not been investigated. With tightly controlled isotopic, spectroscopic, and chromatographic experiments, we quantified and analyzed the mechanisms of N acquisition from iron oxide mineral-associated proteins by Paxillus involutus, a widespread ECM fungus in boreal forests. The fungus acquired N from the mineral-associated proteins. The collective results indicated a proteolytic mechanism involving formation of the crucial enzyme-substrate complexes at the mineral surfaces. Hence, the enzymes hydrolyzed the mineral-associated proteins without initial desorption of the proteins. The proteolytic activity was suppressed by adsorption of proteases to the mineral particles. This process was counteracted by fungal secretion of mineral-surface-reactive compounds that decreased the protease-mineral interactions and thereby promoted the formation of enzyme-substrate complexes. The ability of ECM fungi to simultaneously generate extracellular proteases and surface-reactive metabolites suggests that they can play an important role in unlocking the large N pool of mineral-associated proteins to trees in boreal forests.
Substances chimiques
Minerals
0
Soil
0
Nitrogen
N762921K75
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
697-711Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2020 The Authors. New Phytologist © 2020 New Phytologist Trust.
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