The Implication of Spatial Statistics in Human Mesenchymal Stem Cell Response to Nanotubular Architectures.
bone quality
nanotopography
nanotubes
spatial statistics
stem cells
Journal
International journal of nanomedicine
ISSN: 1178-2013
Titre abrégé: Int J Nanomedicine
Pays: New Zealand
ID NLM: 101263847
Informations de publication
Date de publication:
2020
2020
Historique:
received:
12
11
2019
accepted:
16
02
2020
entrez:
14
4
2020
pubmed:
14
4
2020
medline:
13
6
2020
Statut:
epublish
Résumé
In recent years there has been ample interest in nanoscale modifications of synthetic biomaterials to understand fundamental aspects of cell-surface interactions towards improved biological outcomes. In this study, we aimed at closing in on the effects of nanotubular TiO To bridge this gap, we implemented an extensive characterization of nanotubular surfaces generated by anodization of titanium with a focus on spatial structural variables including eccentricity, nearest neighbour distance (NND) and Voronoi entropy, and associated them to the hMSC response. In addition, we assessed the biological potential of a two-tiered honeycomb nanoarchitecture, which allowed the detection of combinatory effects that this hierarchical structure has on stem cells with respect to conventional nanotubular designs. We have combined experimental techniques, ranging from Scanning Electron (SEM) and Atomic Force (AFM) microscopy to Raman spectroscopy, with computational simulations to characterize and model nanotubular surfaces. We evaluated the cell response at 6 hrs, 1 and 2 days by fluorescence microscopy, as well as bone mineral deposition by Raman spectroscopy, demonstrating substrate-induced differential biological cueing at both the short- and long-term. Our work demonstrates that the nanotube diameter is not sufficient to comprehensively characterize nanotubular surfaces and equally important parameters, such as eccentricity and wall thickness, ought to be included since they all contribute to the overall spatial disorder which, in turn, dictates the overall bioactive potential. We have also demonstrated that nanotubular surfaces affect the quality of bone mineral deposited by differentiated stem cells. Lastly, we closed in on the integrated effects exerted by the superimposition of two dissimilar nanotubular arrays in the honeycomb architecture. This work delineates a novel approach for the characterization of TiO
Identifiants
pubmed: 32280212
doi: 10.2147/IJN.S238280
pii: 238280
pmc: PMC7125340
doi:
Substances chimiques
Minerals
0
Sp7 Transcription Factor
0
SP7 protein, human
0
titanium dioxide
15FIX9V2JP
Titanium
D1JT611TNE
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2151-2169Informations de copyright
© 2020 Steeves et al.
Déclaration de conflit d'intérêts
These authors report no conflicts of interest in this work.
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