KIF4A Promotes Clear Cell Renal Cell Carcinoma (ccRCC) Proliferation in vitro and in vivo.
KIF4A
ccRCC
clear cell renal cell carcinoma
clinicopathological characteristics
prognosis
proliferation
Journal
OncoTargets and therapy
ISSN: 1178-6930
Titre abrégé: Onco Targets Ther
Pays: New Zealand
ID NLM: 101514322
Informations de publication
Date de publication:
2020
2020
Historique:
received:
02
12
2019
accepted:
12
03
2020
entrez:
14
4
2020
pubmed:
14
4
2020
medline:
14
4
2020
Statut:
epublish
Résumé
To evaluate the expression in human clear cell renal cell carcinoma (ccRCC) tissues and explore the effects of kinesin family member 4A (KIF4A) on ccRCC progression. GEPIA was used to evaluate the mRNA levels of KIF4A in human ccRCC tissues from TCGA database, and Immunohistochemistry (IHC) assays were performed to assess its expression in human ccRCC tissues collected in our hospital. The clinical-pathological analysis was performed to explore the correlation with KIF4A expression. The effects of KIF4A on ccRCC cell proliferation were detected through colony formation and MTT assays. Finally, the effects of KIF4A on tumor growth were measured using a mice model. Bioinformation results showed the expression of KIF4A mRNA was upregulated in ccRCC tissues and high expression of KIF4A was related with poor prognosis in ccRCC patients. We also found a high expression of KIF4A in human ccRCC tissues collected in our hospital. We also found its expression level was correlated with clinical characteristics, including T stage (P=0.035*) and lymphatic metastasis (P=0.028*). We further confirmed that knockdown of KIF4A suppressed cell proliferation in HTB-47 and CRL-1932 cells. Furthermore, KIF4A contributes to tumor growth of ccRCC cells in mice. We found the abnormal high expression of KIF4A in human ccRCC tissues and demonstrated that KIF4A could serve as a tumor induction gene.
Identifiants
pubmed: 32280241
doi: 10.2147/OTT.S240734
pii: 240734
pmc: PMC7127824
doi:
Types de publication
Journal Article
Retracted Publication
Langues
eng
Pagination
2667-2676Commentaires et corrections
Type : RetractionIn
Informations de copyright
© 2020 Yang et al.
Déclaration de conflit d'intérêts
The authors declare that they have no competing interests in this work.
Références
PLoS One. 2011;6(10):e25631
pubmed: 22022424
Oncotarget. 2017 Apr 26;8(33):54558-54571
pubmed: 28903364
J Cell Physiol. 2019 Jul;234(7):10360-10371
pubmed: 30417376
Clin Adv Hematol Oncol. 2018 Oct;16(10):677-686
pubmed: 30543598
Actas Urol Esp. 2007 Mar;31(3):233-43
pubmed: 17658151
EMBO J. 2004 Aug 18;23(16):3237-48
pubmed: 15297875
Nat Rev Cancer. 2012 Jul 24;12(8):527-39
pubmed: 22825217
Hum Reprod Update. 2017 Jul 1;23(4):409-420
pubmed: 28431155
Gene. 2018 Dec 15;678:90-99
pubmed: 30081192
J Cell Biol. 2014 Dec 22;207(6):683-93
pubmed: 25512391
Eur J Cancer. 2010 Dec;46(18):3141-8
pubmed: 20709527
Int J Oncol. 2018 Jun;52(6):1912-1922
pubmed: 29620256
Tumour Biol. 2011 Feb;32(1):53-61
pubmed: 20711700
J Cell Biol. 2013 Aug 19;202(4):605-21
pubmed: 23940115
Biochem Biophys Res Commun. 2008 Aug 1;372(3):454-8
pubmed: 18502200
PLoS One. 2013 Dec 30;8(12):e85951
pubmed: 24386490
J Med Genet. 2014 Jul;51(7):487-94
pubmed: 24812067
Urol Oncol. 2018 Mar;36(3):94.e1-94.e14
pubmed: 29169846
Med Oncol. 2018 May 4;35(6):85
pubmed: 29728867
J Cell Biochem. 2019 Feb;120(2):1492-1502
pubmed: 30304555
Oncogene. 2018 Feb 15;37(7):833-838
pubmed: 29059174
J Vis Exp. 2017 Apr 12;(122):
pubmed: 28448047
Int J Oncol. 2006 Oct;29(4):799-827
pubmed: 16964377
Biomed Rep. 2018 Apr;8(4):343-349
pubmed: 29541455
Exp Cell Res. 2015 May 15;334(1):16-25
pubmed: 25724902
Oncogene. 2012 Mar 29;31(13):1683-94
pubmed: 21909141
Curr Opin Urol. 2005 Sep;15(5):289-97
pubmed: 16093851
Cell Death Dis. 2018 Apr 30;9(5):477
pubmed: 29706624
Oncol Lett. 2018 Feb;15(2):2188-2194
pubmed: 29434924
Aging Dis. 2018 Aug 1;9(4):623-633
pubmed: 30090651
Clin Cancer Res. 2007 Nov 15;13(22 Pt 1):6624-31
pubmed: 18006763
Mol Cells. 2014 Nov;37(11):812-8
pubmed: 25377255
J Cell Biol. 2004 Aug 30;166(5):613-20
pubmed: 15326200
Cancer Discov. 2017 Nov;7(11):1221-1223
pubmed: 29097620
Chin Med J (Engl). 2015 Aug 5;128(15):2026-33
pubmed: 26228213
Am J Clin Pathol. 2015 Apr;143(4):492-9
pubmed: 25780000