Glycan characterization of pregnancy-specific glycoprotein 1 and its identification as a novel Galectin-1 ligand.
galectin
glycosylation
pregnancy specific glycoproteins
Journal
Glycobiology
ISSN: 1460-2423
Titre abrégé: Glycobiology
Pays: England
ID NLM: 9104124
Informations de publication
Date de publication:
21 10 2020
21 10 2020
Historique:
received:
09
10
2019
revised:
23
03
2020
accepted:
08
04
2020
pubmed:
14
4
2020
medline:
3
11
2021
entrez:
14
4
2020
Statut:
ppublish
Résumé
Pregnancy-specific beta 1 glycoprotein (PSG1) is secreted from trophoblast cells of the human placenta in increasing concentrations as pregnancy progresses, becoming one of the most abundant proteins in maternal serum in the third trimester. PSG1 has seven potential N-linked glycosylation sites across its four domains. We carried out glycomic and glycoproteomic studies to characterize the glycan composition of PSG1 purified from serum of pregnant women and identified the presence of complex N-glycans containing poly LacNAc epitopes with α2,3 sialyation at four sites. Using different techniques, we explored whether PSG1 can bind to galectin-1 (Gal-1) as these two proteins were previously shown to participate in processes required for a successful pregnancy. We confirmed that PSG1 binds to Gal-1 in a carbohydrate-dependent manner with an affinity of the interaction of 0.13 μM. In addition, we determined that out of the three N-glycosylation-carrying domains, only the N and A2 domains of recombinant PSG1 interact with Gal-1. Lastly, we observed that the interaction between PSG1 and Gal-1 protects this lectin from oxidative inactivation and that PSG1 competes the ability of Gal-1 to bind to some but not all of its glycoprotein ligands.
Identifiants
pubmed: 32280962
pii: 5818751
doi: 10.1093/glycob/cwaa034
pmc: PMC7581653
doi:
Substances chimiques
Galectin 1
0
Ligands
0
Polysaccharides
0
Pregnancy-Specific beta 1-Glycoproteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
895-909Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/F008309/1
Pays : United Kingdom
Organisme : NIAID NIH HHS
ID : R21 AI146368
Pays : United States
Organisme : NCI NIH HHS
ID : U01 CA225644
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI120918
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM070589
Pays : United States
Informations de copyright
Published by Oxford University Press 2020. This work is written by (a) US Government employee(s) and is in the public domain in the US.
Références
J Biol Chem. 2013 Jan 25;288(4):2571-9
pubmed: 23223580
Fetal Diagn Ther. 2008;24(3):230-6
pubmed: 18753763
J Immunol. 2006 Oct 15;177(8):5328-36
pubmed: 17015718
BMC Biol. 2010 Feb 04;8:12
pubmed: 20132533
J Biol Chem. 2015 Feb 13;290(7):4422-31
pubmed: 25548275
Bibl Anat. 1982;(22):87-92
pubmed: 7126157
Nat Immunol. 2007 Aug;8(8):825-34
pubmed: 17589510
Proteins. 2000 Aug 15;40(3):378-88
pubmed: 10861929
J Immunol. 2010 Oct 15;185(8):4659-72
pubmed: 20844192
Hum Reprod. 1998 Mar;13(3):730-5
pubmed: 9572443
Cell Death Differ. 2011 May;18(5):806-16
pubmed: 21113146
Biochemistry. 1982 Oct 26;21(22):5523-8
pubmed: 6983365
Glycobiology. 2012 Oct;22(10):1374-86
pubmed: 22752006
Front Immunol. 2019 May 29;10:1166
pubmed: 31231368
Hum Reprod Update. 2014 Mar-Apr;20(2):175-93
pubmed: 24077937
Cells Tissues Organs. 2007;185(4):269-84
pubmed: 17587801
Placenta. 2015 Apr;36(4):357-64
pubmed: 25659296
Biochimie. 2015 Sep;116:8-16
pubmed: 26116885
Nature. 2015 Oct 1;526(7571):75-81
pubmed: 26432246
J Immunol Res. 2018 Jan 31;2018:9517842
pubmed: 29651447
Placenta. 2007 Apr;28(4):270-7
pubmed: 16793133
Biochemistry. 2010 Sep 7;49(35):7652-8
pubmed: 20666428
BMC Genomics. 2017 Sep 11;18(1):717
pubmed: 28893191
J Biol Chem. 1995 Mar 10;270(10):5198-206
pubmed: 7890630
Br J Obstet Gynaecol. 1976 Oct;83(10):775-9
pubmed: 990216
Cells. 2019 Oct 31;8(11):
pubmed: 31683744
Glycobiology. 1994 Oct;4(5):567-75
pubmed: 7881170
Sci Rep. 2018 Jan 17;8(1):980
pubmed: 29343868
Mol Hum Reprod. 2018 Dec 1;24(12):602-612
pubmed: 30371828
Biochemistry. 2010 Nov 9;49(44):9518-32
pubmed: 20873803
Placenta. 2004 Nov;25(10):797-802
pubmed: 15451194
Int J Dev Biol. 2014;58(2-4):273-80
pubmed: 25023693
Hypertens Res. 2018 Jan;41(1):45-52
pubmed: 28978981
Proteomics. 2016 Dec;16(24):3111-3125
pubmed: 27582340
Hum Reprod. 2015 Sep;30(9):2064-75
pubmed: 26109616
J Mol Biol. 2004 Oct 29;343(4):957-70
pubmed: 15476813
J Biol Chem. 2013 Aug 23;288(34):24394-409
pubmed: 23824193
Mol Cell Proteomics. 2016 Jun;15(6):1857-66
pubmed: 26929217
Nat Med. 2007 Dec;13(12):1450-7
pubmed: 18026113
Front Immunol. 2019 Jan 10;9:3142
pubmed: 30687334
J Biol Chem. 2009 Feb 20;284(8):4989-99
pubmed: 19103599
Placenta. 2007 Nov-Dec;28(11-12):1165-73
pubmed: 17664004
Oncogene. 2008 Jun 12;27(26):3746-53
pubmed: 18223683
Alcohol Alcohol Suppl. 1987;1:473-6
pubmed: 2447904
Glycobiology. 1995 Mar;5(2):255-61
pubmed: 7780201
PLoS One. 2013 Apr 16;8(4):e61701
pubmed: 23613906
J Pathol Transl Med. 2015 May;49(3):181-208
pubmed: 26018511
Biochemistry. 2005 Sep 20;44(37):12564-71
pubmed: 16156668
BMC Genomics. 2005 Jan 12;6:4
pubmed: 15647114
Biochem Biophys Res Commun. 2003 Oct 24;310(3):1010-6
pubmed: 14550305
Am J Reprod Immunol. 2001 Apr;45(4):205-16
pubmed: 11327547
Biol Reprod. 2001 Jan;64(1):90-9
pubmed: 11133662
Mol Hum Reprod. 2013 Jan;19(1):43-53
pubmed: 23002109
J Immunol. 2001 Nov 15;167(10):5697-707
pubmed: 11698442
Proc Natl Acad Sci U S A. 2009 Jun 16;106(24):9731-6
pubmed: 19497882
Diabetes. 2011 Mar;60(3):909-17
pubmed: 21300843
J Reprod Immunol. 2016 Apr;114:38-43
pubmed: 26956510
Placenta. 2018 Jun;66:17-25
pubmed: 29884298
Mucosal Immunol. 2014 Mar;7(2):348-58
pubmed: 23945545
Arch Gynecol. 1979;227(4):327-36
pubmed: 316310
J Biol Chem. 1992 May 25;267(15):10342-7
pubmed: 1587821
Reprod Biomed Online. 2012 Jan;24(1):116-22
pubmed: 22119323
J Clin Invest. 1987 Nov;80(5):1342-9
pubmed: 3680500
Mol Cell Biol. 2006 Apr;26(8):3181-93
pubmed: 16581792
Oncotarget. 2017 Aug 7;8(41):70653-70668
pubmed: 29050309
Proc Natl Acad Sci U S A. 2013 Jul 9;110(28):11451-6
pubmed: 23798433
Science. 2018 Jun 8;360(6393):1133-1136
pubmed: 29880692
Eur J Immunol. 2012 Jun;42(6):1573-84
pubmed: 22678910
Sci Rep. 2016 Sep 08;6:32956
pubmed: 27604319
Proc Natl Acad Sci U S A. 1994 Feb 15;91(4):1428-32
pubmed: 8108426
Placenta. 2009 Nov;30(11):939-48
pubmed: 19766308
J Biol Chem. 2010 Feb 19;285(8):5759-75
pubmed: 19951948
PLoS One. 2018 Mar 22;13(3):e0194870
pubmed: 29566059
PLoS One. 2011;6(12):e28514
pubmed: 22174828
J Biol Chem. 2007 May 25;282(21):15700-8
pubmed: 17383961
Am J Reprod Immunol. 2008 Mar;59(3):251-8
pubmed: 18275518
J Biol Chem. 2011 Mar 4;286(9):7577-86
pubmed: 21193412
J Immunol. 2000 Sep 1;165(5):2331-4
pubmed: 10946254
PLoS One. 2013;8(2):e57491
pubmed: 23469002
Biochim Biophys Acta. 2002 Sep 19;1572(2-3):232-54
pubmed: 12223272
Am J Reprod Immunol. 2013 Apr;69(4):383-94
pubmed: 23432664
J Biol Chem. 2008 Apr 11;283(15):10109-23
pubmed: 18216021
J Matern Fetal Neonatal Med. 2015 Mar;28(5):558-63
pubmed: 24846767
Placenta. 1997 Jul-Aug;18(5-6):433-9
pubmed: 9250706
Mol Cell Biol. 2014 Jul;34(13):2479-87
pubmed: 24752896
Dev Dyn. 1993 Nov;198(3):171-81
pubmed: 8136522
J Matern Fetal Neonatal Med. 2008 Jul;21(7):429-42
pubmed: 18570123
Oncodev Biol Med. 1981;2(3):179-82
pubmed: 6272237
Dev Comp Immunol. 2016 Feb;55:241-252
pubmed: 26429411
Am J Obstet Gynecol. 1976 Jan 1;124(1):51-4
pubmed: 1244746