Suppression of Gq and PLC gene expression has a small effect on quantum bumps in vivo in Periplaneta americana.
Gq protein
Insect photoreceptor
PLC
Phototransduction
Journal
Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology
ISSN: 1432-1351
Titre abrégé: J Comp Physiol A Neuroethol Sens Neural Behav Physiol
Pays: Germany
ID NLM: 101141792
Informations de publication
Date de publication:
07 2020
07 2020
Historique:
received:
22
09
2019
accepted:
28
03
2020
revised:
03
03
2020
pubmed:
15
4
2020
medline:
8
9
2021
entrez:
15
4
2020
Statut:
ppublish
Résumé
Visual signal transmission by Drosophila melanogaster photoreceptors is mediated by a Gq protein that activates a phospholipase C (PLC). Mutations and deficiencies in expression of either of these proteins cause severe defects in phototransduction. Here we investigated whether these proteins are also involved in the cockroach, Periplaneta americana, phototransduction by silencing Gq α-subunit (Gqα) and phosphoinositide-specific phospholipase C (PLC) by RNA interference and observing responses to single photons (quantum bumps, QB). We found (1) non-specific decreases in membrane resistance, membrane capacitance and absolute sensitivity in the photoreceptors of both Gqα and PLC knockdowns, and (2) small changes in QB statistics. Despite significant decreases in expressions of Gq and PLC mRNA, the changes in QB properties were surprisingly modest, with mean latencies increasing by ~ 10%, and without significant decrease in their amplitudes. To better understand our results, we used a mathematical model of the phototransduction cascade. By modifying the Gq and PLC abundances, and diffusion rates for Gq, we found that QB latencies and amplitudes deteriorated noticeably only after large decreases in the protein levels, especially when Gq diffusion was slow. Also, reduction in Gq but not PLC lowered quantum efficiency. These results suggest that expression of the proteins may be redundant.
Identifiants
pubmed: 32285147
doi: 10.1007/s00359-020-01417-7
pii: 10.1007/s00359-020-01417-7
pmc: PMC7314733
doi:
Substances chimiques
Type C Phospholipases
EC 3.1.4.-
GTP-Binding Protein alpha Subunits, Gq-G11
EC 3.6.5.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
597-610Références
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