Gestational diabetes mellitus affects placental iron homeostasis: Mechanism and clinical implications.
Adult
Antigens, CD
/ metabolism
Antioxidants
/ metabolism
Autophagy
/ physiology
Cation Transport Proteins
/ metabolism
Chromatography, Liquid
/ methods
Diabetes, Gestational
/ metabolism
Female
Ferritins
/ metabolism
Fetus
/ metabolism
Homeostasis
/ physiology
Humans
Iron
/ metabolism
Male
Oxidative Stress
/ physiology
Placenta
/ metabolism
Pregnancy
Receptors, Transferrin
/ metabolism
Tandem Mass Spectrometry
/ methods
Trophoblasts
/ metabolism
DMT1
ZIP8
antioxidant treatment
autophagy
ferroportin
immunohistochemistry
iron uptake
oxidative stress
placenta
transferrin receptor
trophoblast cells
Journal
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
ISSN: 1530-6860
Titre abrégé: FASEB J
Pays: United States
ID NLM: 8804484
Informations de publication
Date de publication:
06 2020
06 2020
Historique:
received:
04
12
2019
revised:
07
02
2020
accepted:
02
03
2020
pubmed:
15
4
2020
medline:
20
1
2021
entrez:
15
4
2020
Statut:
ppublish
Résumé
Clinical studies suggest that pregnant women with elevated iron levels are more vulnerable to develop gestational diabetes mellitus (GDM), but the causes and underlying mechanisms are unknown. We hypothesized that hyperglycemia induces cellular stress responses leading to dysregulated placental iron homeostasis. Hence, we compared the expression of genes/proteins involved in iron homeostasis in placentae from GDM and healthy pregnancies (n = 11 each). RT-qPCR and LC-MS/MS analyses revealed differential regulation of iron transporters/receptors (DMT1/FPN1/ZIP8/TfR1), iron sensors (IRP1), iron regulators (HEPC), and iron oxidoreductases (HEPH/Zp). To identify the underlying mechanisms, we adapted BeWo trophoblast cells to normoglycemic (N), hyperglycemic (H), and hyperglycemic-hyperlipidemic (HL) conditions and assessed Fe
Identifiants
pubmed: 32285992
doi: 10.1096/fj.201903054R
doi:
Substances chimiques
Antigens, CD
0
Antioxidants
0
CD71 antigen
0
Cation Transport Proteins
0
Receptors, Transferrin
0
Ferritins
9007-73-2
Iron
E1UOL152H7
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
7311-7329Informations de copyright
© 2020 The Authors. The FASEB Journal published by Wiley Periodicals, Inc. on behalf of Federation of American Societies for Experimental Biology.
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