Soyasapogenol-A targets CARF and results in suppression of tumor growth and metastasis in p53 compromised cancer cells.

Animals Antineoplastic Agents, Phytogenic / pharmacology Apoptosis / drug effects Apoptosis Regulatory Proteins / antagonists & inhibitors Cell Cycle Checkpoints / drug effects Cell Line, Tumor Cell Movement / drug effects Cyclin-Dependent Kinase Inhibitor p21 / metabolism Down-Regulation / drug effects Female Gene Expression Regulation, Neoplastic / drug effects Humans Lung Neoplasms / drug therapy Mice Neoplasm Invasiveness / prevention & control Oleanolic Acid / analogs & derivatives RNA-Binding Proteins / antagonists & inhibitors Saponins / pharmacology Signal Transduction / drug effects Transcriptional Activation / drug effects Tumor Suppressor Protein p53 / deficiency Xenograft Model Antitumor Assays

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
14 04 2020
Historique:
received: 30 01 2019
accepted: 19 03 2020
entrez: 15 4 2020
pubmed: 15 4 2020
medline: 15 12 2020
Statut: epublish

Résumé

We screened some phytochemicals for cytotoxic activity to human cancer cells and identified Soyasapogenol-A (Snol-A) as a potent candidate anti-cancer compound. Interestingly, Soyasapogenin-I (Snin-I) was ineffective. Viability assays endorsed toxicity of Snol-A to a wide variety of cancer cells. Of note, wild type p53 deficient cancer cells (SKOV-3 and Saos-2) also showed potent growth inhibitory effect. Molecular analyses demonstrated that it targets CARF yielding transcriptional upregulation of p21

Identifiants

DOI: 10.1038/s41598-020-62953-5 PMID: 32286347 PMC: PMC7156697
pubmed: 32286347
doi: 10.1038/s41598-020-62953-5
pii: 10.1038/s41598-020-62953-5
pmc: PMC7156697
doi:

Substances chimiques

Antineoplastic Agents, Phytogenic 0
Apoptosis Regulatory Proteins 0
CDKN1A protein, human 0
CDKN2AIP protein, human 0
Cyclin-Dependent Kinase Inhibitor p21 0
RNA-Binding Proteins 0
Saponins 0
TP53 protein, human 0
Tumor Suppressor Protein p53 0
soyasapogenol A 43T6J3O9WL
soyasaponin I 51330-27-9
Oleanolic Acid 6SMK8R7TGJ

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

6323

Références

McQuade, R. M., Stojanovska, V., Abalo, R., Bornstein, J. C. & Nurgali, K. Chemotherapy-Induced Constipation and Diarrhea: Pathophysiology, Current and Emerging Treatments. Front Pharmacol 7, 414, https://doi.org/10.3389/fphar.2016.00414 (2016).
doi: 10.3389/fphar.2016.00414 pubmed: 27857691 pmcid: 5093116
Nurgali, K., Jagoe, R. T. & Abalo, R. Editorial: Adverse Effects of Cancer Chemotherapy: Anything New to Improve Tolerance and Reduce Sequelae? Front Pharmacol 9, 245, https://doi.org/10.3389/fphar.2018.00245 (2018).
doi: 10.3389/fphar.2018.00245 pubmed: 29623040 pmcid: 5874321
Ateba, S. B. et al. Natural Terpenoids Against Female Breast Cancer: A 5-year Recent Research. Curr Med Chem 25, 3162–3213, https://doi.org/10.2174/0929867325666180214110932 (2018).
doi: 10.2174/0929867325666180214110932 pubmed: 29446727
Rejhova, A., Opattova, A., Cumova, A., Sliva, D. & Vodicka, P. Natural compounds and combination therapy in colorectal cancer treatment. Eur J Med Chem 144, 582–594, https://doi.org/10.1016/j.ejmech.2017.12.039 (2018).
doi: 10.1016/j.ejmech.2017.12.039 pubmed: 29289883
Muhammad, J. et al. Network Pharmacology: Exploring the Resources and Methodologies. Curr Top Med Chem 18, 949–964, https://doi.org/10.2174/1568026618666180330141351 (2018).
doi: 10.2174/1568026618666180330141351 pubmed: 29600765
de Silva, M. B. & Tencomnao, T. The protective effect of some Thai plants and their bioactive compounds in UV light-induced skin carcinogenesis. J Photochem Photobiol B 185, 80–89, https://doi.org/10.1016/j.jphotobiol.2018.04.046 (2018).
doi: 10.1016/j.jphotobiol.2018.04.046 pubmed: 29879588
Maiuthed, A., Chantarawong, W. & Chanvorachote, P. Lung Cancer Stem Cells and Cancer Stem Cell-targeting Natural Compounds. Anticancer Res 38, 3797–3809, https://doi.org/10.21873/anticanres.12663 (2018).
doi: 10.21873/anticanres.12663 pubmed: 29970499
Xu, R., Fazio, G. C. & Matsuda, S. P. On the origins of triterpenoid skeletal diversity. Phytochemistry 65, 261–291 (2004).
doi: 10.1016/j.phytochem.2003.11.014
Rai, S. N. et al. Anti-inflammatory Activity of Ursolic Acid in MPTP-Induced Parkinsonian Mouse Model. Neurotox Res 36, 452–46,  https://doi.org/10.1007/s12640-019-00038-6 (2019).
Jehangir, A., Shahzad, M., Shahid, K., Waheed, A. & Ayub, F. Zinc and iron complexes of oleanolic acid, (OA) attenuate allergic airway inflammation in rats. Inflammopharmacology 27, 1179–1192,  https://doi.org/10.1007/s10787-019-00597-2 (2019).
doi: 10.1007/s10787-019-00597-2 pubmed: 31069605
Wang, L. et al. Chikusetsu saponin IVa attenuates isoprenaline-induced myocardial fibrosis in mice through activation autophagy mediated by AMPK/mTOR/ULK1 signaling. Phytomedicine 58, 152764, https://doi.org/10.1016/j.phymed.2018.11.024 (2018).
doi: 10.1016/j.phymed.2018.11.024 pubmed: 31005723
Mohammed, A., Victoria Awolola, G., Ibrahim, M. A., Anthony Koorbanally, N. & Islam, M. S. Oleanolic acid as a potential antidiabetic component of Xylopia aethiopica (Dunal) A. Rich. (Annonaceae) fruit: bioassay guided isolation and molecular docking studies. Nat Prod Res, 1–4, https://doi.org/10.1080/14786419.2019.1596094 (2019).
Sharma, H., Kumar, P., Deshmukh, R. R., Bishayee, A. & Kumar, S. Pentacyclic triterpenes: New tools to fight metabolic syndrome. Phytomedicine 50, 166–177, https://doi.org/10.1016/j.phymed.2018.09.011 (2018).
doi: 10.1016/j.phymed.2018.09.011 pubmed: 30466975
Zeng, A., Hua, H., Liu, L. & Zhao, J. Betulinic acid induces apoptosis and inhibits metastasis of human colorectal cancer cells in vitro and in vivo. Bioorg Med Chem 27, 2546–2552,  https://doi.org/10.1016/j.bmc.2019.03.033 (2019).
El-Senduny, F. F., Zidane, M. M., Youssef, M. M. & Badria, F. A. An Approach For Treatment of Liver Cancer By Novel Glycyrrhizin Derivative. Anticancer Agents Med Chem 19, 1863–1873,  https://doi.org/10.2174/1871520619666190411114718 (2019).
Messina, M. Soy and Health Update: Evaluation of the Clinical and Epidemiologic Literature. Nutrients 8, https://doi.org/10.3390/nu8120754 (2016).
Rizzo, G. & Baroni, L. Soy, Soy Foods and Their Role in Vegetarian Diets. Nutrients 10, https://doi.org/10.3390/nu10010043 (2018).
Gabriel, A. S., Ninomiya, K. & Uneyama, H. The Role of the Japanese Traditional Diet in Healthy and Sustainable Dietary Patterns around the World. Nutrients 10, https://doi.org/10.3390/nu10020173 (2018).
Kamo, S., Suzuki, S. & Sato, T. The content of soyasaponin and soyasapogenol in soy foods and their estimated intake in the Japanese. Food Sci Nutr 2, 289–297, https://doi.org/10.1002/fsn3.107 (2014).
doi: 10.1002/fsn3.107 pubmed: 24936299 pmcid: 4048615
Yoshiki, Y., Kudou, S. & Okubo, K. Relationship between chemical structures and biological activities of triterpenoid saponins from soybean. Biosci Biotechnol Biochem 62, 2291–2299, https://doi.org/10.1271/bbb.62.2291 (1998).
doi: 10.1271/bbb.62.2291 pubmed: 9972256
Shiraiwa M1, H. K. & Okubo K. Composition and structure of “group B saponin” in soybean seed. Agric. Biol. Chem. 55(4), 911–917, https://doi.org/10.1271/bbb1961.55.911 (1991).
Zhang, W. & Popovich, D. G. Chemical and biological characterization of oleanane triterpenoids from soy. Molecules 14, 2959–2975, https://doi.org/10.3390/molecules14082959 (2009).
doi: 10.3390/molecules14082959 pubmed: 19701138 pmcid: 6255087
Guang, C., Chen, J., Sang, S. & Cheng, S. Biological functionality of soyasaponins and soyasapogenols. J Agric Food Chem 62, 8247–8255, https://doi.org/10.1021/jf503047a (2014).
doi: 10.1021/jf503047a pubmed: 25074337
Zhang, W., Yeo, M. C., Tang, F. Y. & Popovich, D. G. Bioactive responses of Hep-G2 cells to soyasaponin extracts differs with respect to extraction conditions. Food Chem Toxicol 47, 2202–2208, https://doi.org/10.1016/j.fct.2009.06.006 (2009).
doi: 10.1016/j.fct.2009.06.006 pubmed: 19520140
Rao, D. M. G. A. V. Soyasaponins: The Relationship Between Chemical Structure and Colon Anticarcinogenic Activity. Nutrition and Cancer 47(1), 24–33, https://doi.org/10.1207/s15327914nc4701_3 (2003).
doi: 10.1207/s15327914nc4701_3 pubmed: 14769534
Xiao, J. X., Huang, G. Q. & Zhang, S. H. Soyasaponins inhibit the proliferation of Hela cells by inducing apoptosis. Exp Toxicol Pathol 59, 35–42, https://doi.org/10.1016/j.etp.2007.02.004 (2007).
doi: 10.1016/j.etp.2007.02.004 pubmed: 17582751
Zhang, W. & Popovich, D. G. Effect of soyasapogenol A and soyasapogenol B concentrated extracts on HEP-G2 cell proliferation and apoptosis. J Agric Food Chem 56, 2603–2608, https://doi.org/10.1021/jf0731550 (2008).
doi: 10.1021/jf0731550 pubmed: 18361499
Zhang, W. & Popovich, D. G. Group B oleanane triterpenoid extract containing soyasaponins I and III from soy flour induces apoptosis in Hep-G2 cells. J Agric Food Chem 58, 5315–5319, https://doi.org/10.1021/jf9037979 (2010).
doi: 10.1021/jf9037979 pubmed: 20345167
Hasan, M. K. et al. CARF is a novel protein that cooperates with mouse p19ARF (human p14ARF) in activating p53. J Biol Chem 277, 37765–37770, https://doi.org/10.1074/jbc.M204177200 (2002).
doi: 10.1074/jbc.M204177200 pubmed: 12154087
Hasan, M. K. et al. Alternative reading frame protein (ARF)-independent function of CARF (collaborator of ARF) involves its interactions with p53: evidence for a novel p53-activation pathway and its negative feedback control. Biochem J 380, 605–610, https://doi.org/10.1042/BJ20040337 (2004).
doi: 10.1042/BJ20040337 pubmed: 15109303 pmcid: 1224239
Hasan, M. K. et al. CARF (collaborator of ARF) interacts with HDM2: evidence for a novel regulatory feedback regulation of CARF-p53-HDM2-p21WAF1 pathway. Int J Oncol 32, 663–671,  https://doi.org/10.3892/ijo.32.3.663 (2008).
pubmed: 18292944
Hasan, K. et al. CARF Is a vital dual regulator of cellular senescence and apoptosis. J Biol Chem 284, 1664–1672, https://doi.org/10.1074/jbc.M805778200 (2009).
doi: 10.1074/jbc.M805778200 pubmed: 19001376
Cheung, C. T., Kaul, S. C. & Wadhwa, R. Molecular bridging of aging and cancer: A CARF link. Ann N Y Acad Sci 1197, 129–133, https://doi.org/10.1111/j.1749-6632.2009.05392.x (2010).
doi: 10.1111/j.1749-6632.2009.05392.x pubmed: 20536841
Cheung, C. T., Singh, R., Kalra, R. S., Kaul, S. C. & Wadhwa, R. Collaborator of ARF (CARF) regulates proliferative fate of human cells by dose-dependent regulation of DNA damage signaling. J Biol Chem 289, 18258–18269, https://doi.org/10.1074/jbc.M114.547208 (2014).
doi: 10.1074/jbc.M114.547208 pubmed: 24825908 pmcid: 4140265
Cheung, C. T. et al. Molecular characterization of apoptosis induced by CARF silencing in human cancer cells. Cell Death Differ 18, 589–601, https://doi.org/10.1038/cdd.2010.129 (2011).
doi: 10.1038/cdd.2010.129 pubmed: 21052095
Kalra, R. S. et al. CARF (Collaborator of ARF) overexpression in p53-deficient cells promotes carcinogenesis. Mol Oncol 9, 1877–1889, https://doi.org/10.1016/j.molonc.2015.07.003 (2015).
doi: 10.1016/j.molonc.2015.07.003 pubmed: 26278998 pmcid: 5528716
Kalra, R. S. et al. CARF enrichment promotes epithelial-mesenchymal transition via Wnt/beta-catenin signaling: its clinical relevance and potential as a therapeutic target. Oncogenesis 7, 39, https://doi.org/10.1038/s41389-018-0048-4 (2018).
doi: 10.1038/s41389-018-0048-4 pubmed: 29748568 pmcid: 5945778
Diaza, R. G. et al. Comparison of in silico tools for evaluating rat oral acute toxicity. SAR QSAR Environ Res 26, 1–27, https://doi.org/10.1080/1062936X.2014.977819 (2015).
doi: 10.1080/1062936X.2014.977819 pubmed: 25567032
Khan, T. et al. Computer-aided drug design and virtual screening of targeted combinatorial libraries of mixed-ligand transition metal complexes of 2-butanone thiosemicarbazone. Comput Biol Chem 75, 178–195, https://doi.org/10.1016/j.compbiolchem.2018.05.008 (2018).
doi: 10.1016/j.compbiolchem.2018.05.008 pubmed: 29883916
Popiolkiewicz, J., Polkowski, K., Skierski, J. S. & Mazurek, A. P. In vitro toxicity evaluation in the development of new anticancer drugs-genistein glycosides. Cancer Lett 229, 67–75, https://doi.org/10.1016/j.canlet.2005.01.014 (2005).
doi: 10.1016/j.canlet.2005.01.014 pubmed: 16157220
Singh, R. et al. Molecular characterization of collaborator of ARF (CARF) as a DNA damage response and cell cycle checkpoint regulatory protein. Exp Cell Res 322, 324–334, https://doi.org/10.1016/j.yexcr.2014.01.022 (2014).
doi: 10.1016/j.yexcr.2014.01.022 pubmed: 24485912
Wadhwa, R., Kalra, R. S. & Kaul, S. C. CARF is a multi-module regulator of cell proliferation and a molecular bridge between cellular senescence and carcinogenesis. Mech Ageing Dev 166, 64–68, https://doi.org/10.1016/j.mad.2017.07.008 (2017).
doi: 10.1016/j.mad.2017.07.008 pubmed: 28754531
Pei, Y., Zhao, H., Du, X. & Li, J. Apoptosis effects on human esophageal cancer cells by soyasaponin Bb and its machanism. Wei Sheng Yan Jiu 39, 444–446 (2010).
pubmed: 20726234
Chang, W. W., Yu, C. Y., Lin, T. W., Wang, P. H. & Tsai, Y. C. Soyasaponin I decreases the expression of alpha2,3-linked sialic acid on the cell surface and suppresses the metastatic potential of B16F10 melanoma cells. Biochem Biophys Res Commun 341, 614–619, https://doi.org/10.1016/j.bbrc.2005.12.216 (2006).
doi: 10.1016/j.bbrc.2005.12.216 pubmed: 16427612
Hsu, C. C. et al. Soyasaponin-I-modified invasive behavior of cancer by changing cell surface sialic acids. Gynecol Oncol 96, 415–422, https://doi.org/10.1016/j.ygyno.2004.10.010 (2005).
doi: 10.1016/j.ygyno.2004.10.010 pubmed: 15661230
Ellington, A. A., Berhow, M. A. & Singletary, K. W. Inhibition of Akt signaling and enhanced ERK1/2 activity are involved in induction of macroautophagy by triterpenoid B-group soyasaponins in colon cancer cells. Carcinogenesis 27, 298–306, https://doi.org/10.1093/carcin/bgi214 (2006).
doi: 10.1093/carcin/bgi214 pubmed: 16113053
Marcela, G. M., Eva, R. G., Del Carmen, R. M. & Rosalva, M. E. Evaluation of the Antioxidant and Antiproliferative Effects of Three Peptide Fractions of Germinated Soybeans on Breast and Cervical Cancer Cell Lines. Plant Foods Hum Nutr 71, 368–374, https://doi.org/10.1007/s11130-016-0568-z (2016).
doi: 10.1007/s11130-016-0568-z pubmed: 27401682
MacDonald, R. S. et al. Environmental influences on isoflavones and saponins in soybeans and their role in colon cancer. J Nutr 135, 1239–1242, https://doi.org/10.1093/jn/135.5.1239 (2005).
doi: 10.1093/jn/135.5.1239 pubmed: 15867311
Toyomura, K. & Kono, S. Soybeans, Soy Foods, Isoflavones and Risk of Colorectal Cancer: a Review of Experimental and Epidemiological Data. Asian Pac J Cancer Prev 3, 125–132 (2002).
pubmed: 12718590
Barnes, S., Grubbs, C., Setchell, K. D. & Carlson, J. Soybeans inhibit mammary tumors in models of breast cancer. Prog Clin Biol Res 347, 239–253 (1990).
pubmed: 2217394
Li, L. et al. Tumor suppressor activity of miR-451: Identification of CARF as a new target. Sci Rep 8, 375, https://doi.org/10.1038/s41598-017-18559-5 (2018).
doi: 10.1038/s41598-017-18559-5 pubmed: 29321561 pmcid: 5762681
Chaudhary, A. et al. 2,3-Dihydro-3beta-methoxy Withaferin-A Protects Normal Cells against Stress: Molecular Evidence of Its Potent Cytoprotective Activity. J Nat Prod 80, 2756–2760, https://doi.org/10.1021/acs.jnatprod.7b00573 (2017).
doi: 10.1021/acs.jnatprod.7b00573 pubmed: 29043807

Auteurs

Amr Omar (A)

AIST-INDIA DAILAB, DBT-AIST International Center for Translational & Environmental Research (DAICENTER), National Institute of Advanced Industrial Science & Technology (AIST), Tsukuba, 305 8565, Japan.
School of Integrative & Global Majors, University of Tsukuba, Tsukuba, Japan.

Rajkumar Singh Kalra (RS)

AIST-INDIA DAILAB, DBT-AIST International Center for Translational & Environmental Research (DAICENTER), National Institute of Advanced Industrial Science & Technology (AIST), Tsukuba, 305 8565, Japan.

Jayarani Putri (J)

AIST-INDIA DAILAB, DBT-AIST International Center for Translational & Environmental Research (DAICENTER), National Institute of Advanced Industrial Science & Technology (AIST), Tsukuba, 305 8565, Japan.

Ahmed Elwakeel (A)

AIST-INDIA DAILAB, DBT-AIST International Center for Translational & Environmental Research (DAICENTER), National Institute of Advanced Industrial Science & Technology (AIST), Tsukuba, 305 8565, Japan.
School of Integrative & Global Majors, University of Tsukuba, Tsukuba, Japan.
ORCID: 0000-0001-5195-5219

Sunil C Kaul (SC)

AIST-INDIA DAILAB, DBT-AIST International Center for Translational & Environmental Research (DAICENTER), National Institute of Advanced Industrial Science & Technology (AIST), Tsukuba, 305 8565, Japan. s-kaul@aist.go.jp.
School of Integrative & Global Majors, University of Tsukuba, Tsukuba, Japan. s-kaul@aist.go.jp.

Renu Wadhwa (R)

AIST-INDIA DAILAB, DBT-AIST International Center for Translational & Environmental Research (DAICENTER), National Institute of Advanced Industrial Science & Technology (AIST), Tsukuba, 305 8565, Japan. renu-wadhwa@aist.go.jp.
School of Integrative & Global Majors, University of Tsukuba, Tsukuba, Japan. renu-wadhwa@aist.go.jp.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Soyasapogenol-A targets CARF and results in...
questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Utilisations thérapeutiques › Antinéoplasiques › Antinéoplasiques d'origine végétale › Soyasapogenol-A targets CARF and results in...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Mort cellulaire › Mort cellulaire régulée › Apoptose › Soyasapogenol-A targets CARF and results in...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protéines régulatrices de l'apoptose › Soyasapogenol-A targets CARF and results in...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Cycle cellulaire › Points de contrôle du cycle cellulaire › Soyasapogenol-A targets CARF and results in...
questionsmedicales.fr › Cellules › Cellules cultivées › Cellules cancéreuses en culture › Lignée cellulaire tumorale › Soyasapogenol-A targets CARF and results in...
questionsmedicales.fr › Phénomènes physiologiques › Mouvement cellulaire › Soyasapogenol-A targets CARF and results in...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines nucléaires › Protéines liant le poly-adp-ribose › Inhibiteur p21 de kinase cycline-dépendante › Soyasapogenol-A targets CARF and results in...
questionsmedicales.fr › Phénomènes physiologiques › Phénomènes pharmacologiques et toxicologiques › Phénomènes pharmacologiques › Régulation négative › Soyasapogenol-A targets CARF and results in...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes tumoraux › Soyasapogenol-A targets CARF and results in...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Soyasapogenol-A targets CARF and results in...
questionsmedicales.fr › Maladies de l'appareil respiratoire › Tumeurs de l'appareil respiratoire › Tumeurs du poumon › Soyasapogenol-A targets CARF and results in...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Soyasapogenol-A targets CARF and results in...
questionsmedicales.fr › États, signes et symptômes pathologiques › Processus pathologiques › Processus néoplasiques › Invasion tumorale › Soyasapogenol-A targets CARF and results in...
questionsmedicales.fr › Composés chimiques organiques › Hydrocarbures › Terpènes › Triterpènes › Acide oléanolique › Soyasapogenol-A targets CARF and results in...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Nucléoprotéines › Protéines de liaison à l'ARN › Soyasapogenol-A targets CARF and results in...
questionsmedicales.fr › Glucides › Hétérosides › Saponines › Soyasapogenol-A targets CARF and results in...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Transduction du signal › Soyasapogenol-A targets CARF and results in...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Activation de la transcription › Soyasapogenol-A targets CARF and results in...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Phosphoprotéines › Protéine p53 suppresseur de tumeur › Soyasapogenol-A targets CARF and results in...
questionsmedicales.fr › Techniques d'investigation › Transplantation tumorale › Tests d'activité antitumorale sur modèle de xénogreffe › Soyasapogenol-A targets CARF and results in...