Regulatory effects of Ganoderma atrum polysaccharides on LPS-induced inflammatory macrophages model and intestinal-like Caco-2/macrophages co-culture inflammation model.
Animals
Caco-2 Cells
Coculture Techniques
Enzyme-Linked Immunosorbent Assay
Ganoderma
/ chemistry
Humans
Inflammation
/ prevention & control
Kelch-Like ECH-Associated Protein 1
/ metabolism
Lipopolysaccharides
/ toxicity
Macrophages
/ drug effects
Mice
NF-E2-Related Factor 2
/ metabolism
Polysaccharides
/ pharmacology
RAW 264.7 Cells
Anti-inflammatory effects
Caco-2/macrophages co-culture model
Ganoderma atrum polysaccharides
LPS
Macrophages
Journal
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
ISSN: 1873-6351
Titre abrégé: Food Chem Toxicol
Pays: England
ID NLM: 8207483
Informations de publication
Date de publication:
Jun 2020
Jun 2020
Historique:
received:
19
09
2019
revised:
10
03
2020
accepted:
03
04
2020
pubmed:
15
4
2020
medline:
9
2
2021
entrez:
15
4
2020
Statut:
ppublish
Résumé
Lipopolysaccharide (LPS)-induced inflammatory macrophages model and intestinal-like Caco-2/macrophages co-culture inflammation model were established to evaluate the anti-inflammatory effect and underlying mechanism of Ganoderma atrum polysaccharides (PSG-1). It was found that PSG-1 reduced LPS-induced secretion of pro-inflammatory cytokine (TNF-α, IL-6 and IL-1β), ROS levels, and inhibited the expression of COX-2 in LPS-stimulated inflammatory macrophages model and intestinal-like Caco-2/macrophages co-culture inflammation model. Furthermore, PSG-1 suppressed the LPS-induced activation of MAPKs signaling pathways, and regulated oxidative stress by activating the Nrf2/Keap1 signaling pathways. These above results indicated that PSG-1 not only has a direct anti-inflammatory effect in LPS-induced inflammatory macrophages model, but also has an indirect anti-inflammatory effect in intestinal-like Caco-2/macrophages co-culture inflammation model. These findings provide new insight of the mechanism underlying the anti-inflammatory activities of PSG-1, and facilitated the expansion of the application of PSG-1 in natural functional food.
Identifiants
pubmed: 32289334
pii: S0278-6915(20)30209-X
doi: 10.1016/j.fct.2020.111321
pii:
doi:
Substances chimiques
Kelch-Like ECH-Associated Protein 1
0
Lipopolysaccharides
0
NF-E2-Related Factor 2
0
Polysaccharides
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
111321Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.