Enzyme Immobilization on Maghemite Nanoparticles with Improved Catalytic Activity: An Electrochemical Study for Xanthine.
catalytic properties
enzyme immobilization
metal nanoparticles
xanthine oxidases
Journal
Materials (Basel, Switzerland)
ISSN: 1996-1944
Titre abrégé: Materials (Basel)
Pays: Switzerland
ID NLM: 101555929
Informations de publication
Date de publication:
10 Apr 2020
10 Apr 2020
Historique:
received:
09
03
2020
revised:
01
04
2020
accepted:
08
04
2020
entrez:
16
4
2020
pubmed:
16
4
2020
medline:
16
4
2020
Statut:
epublish
Résumé
Generally, enzyme immobilization on nanoparticles leads to nano-conjugates presenting partially preserved, or even absent, biological properties. Notwithstanding, recent research demonstrated that the coupling to nanomaterials can improve the activity of immobilized enzymes. Herein, xanthine oxidase (XO) was immobilized by self-assembly on peculiar naked iron oxide nanoparticles (surface active maghemite nanoparticles, SAMNs). The catalytic activity of the nanostructured conjugate (SAMN@XO) was assessed by optical spectroscopy and compared to the parent enzyme. SAMN@XO revealed improved catalytic features with respect to the parent enzyme and was applied for the electrochemical studies of xanthine. The present example supports the nascent knowledge concerning protein conjugation to nanoparticle as a means for the modulation of biological activity.
Identifiants
pubmed: 32290055
pii: ma13071776
doi: 10.3390/ma13071776
pmc: PMC7179010
pii:
doi:
Types de publication
Journal Article
Langues
eng
Références
J R Soc Interface. 2015 Jan 6;12(102):20140891
pubmed: 25392397
Proc Natl Acad Sci U S A. 2000 Sep 26;97(20):10723-8
pubmed: 11005854
Anal Biochem. 1976 May 7;72:248-54
pubmed: 942051
Curr Med Chem. 2018 Feb 12;25(4):540-555
pubmed: 28618993
Biochem J. 1973 Feb;131(2):191-8
pubmed: 4352904
Langmuir. 2012 Oct 30;28(43):15392-401
pubmed: 23057670
Nanoscale. 2011 Sep 1;3(9):3721-30
pubmed: 21792451
Chem Rev. 2005 Apr;105(4):1025-102
pubmed: 15826010
Talanta. 2017 May 15;167:286-295
pubmed: 28340722
J Biol Inorg Chem. 2015 Mar;20(2):183-94
pubmed: 25425163
J Biol Chem. 2008 Sep 26;283(39):26297-301
pubmed: 18544537
Enzyme Microb Technol. 2014 Apr 10;57:55-62
pubmed: 24629268
Curr Opin Chem Biol. 2010 Dec;14(6):828-34
pubmed: 21035376
Anal Bioanal Chem. 2018 May;410(12):2949-2959
pubmed: 29532191
Anal Bioanal Chem. 2008 Feb;390(3):971-7
pubmed: 18060389
Acta Biomater. 2012 Jul;8(6):2068-76
pubmed: 22342825
Biosens Bioelectron. 2011 Apr 15;26(8):3420-6
pubmed: 21324666
Chem Rev. 2011 Sep 14;111(9):5610-37
pubmed: 21688848
Adv Med Sci. 2012;57(2):196-207
pubmed: 23154427
Curr Opin Biotechnol. 2015 Aug;34:242-50
pubmed: 25957941
J Biol Inorg Chem. 2015 Mar;20(2):323-35
pubmed: 25698503
Amino Acids. 2019 Apr;51(4):679-690
pubmed: 30725223
Talanta. 2007 Dec 15;74(3):434-9
pubmed: 18371660