Revisiting Proinsulin Processing: Evidence That Human β-Cells Process Proinsulin With Prohormone Convertase (PC) 1/3 but Not PC2.


Journal

Diabetes
ISSN: 1939-327X
Titre abrégé: Diabetes
Pays: United States
ID NLM: 0372763

Informations de publication

Date de publication:
07 2020
Historique:
received: 13 03 2019
accepted: 03 04 2020
pubmed: 16 4 2020
medline: 31 12 2020
entrez: 16 4 2020
Statut: ppublish

Résumé

Insulin is first produced in pancreatic β-cells as the precursor prohormone proinsulin. Defective proinsulin processing has been implicated in the pathogenesis of both type 1 and type 2 diabetes. Though there is substantial evidence that mouse β-cells process proinsulin using prohormone convertase 1/3 (PC1/3) and then prohormone convertase 2 (PC2), this finding has not been verified in human β-cells. Immunofluorescence with validated antibodies revealed that there was no detectable PC2 immunoreactivity in human β-cells and little

Identifiants

pubmed: 32291281
pii: db19-0276
doi: 10.2337/db19-0276
doi:

Substances chimiques

Proinsulin 9035-68-1
Proprotein Convertase 1 EC 3.4.21.93
PCSK2 protein, human EC 3.4.21.94
Proprotein Convertase 2 EC 3.4.21.94

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1451-1462

Subventions

Organisme : CIHR
Pays : Canada

Informations de copyright

© 2020 by the American Diabetes Association.

Auteurs

Adam Ramzy (A)

Laboratory of Molecular and Cellular Medicine, Department of Cellular and Physiological Sciences, Life Sciences Institute, The University of British Columbia, Vancouver, British Columbia, Canada.

Ali Asadi (A)

Laboratory of Molecular and Cellular Medicine, Department of Cellular and Physiological Sciences, Life Sciences Institute, The University of British Columbia, Vancouver, British Columbia, Canada.

Timothy J Kieffer (TJ)

Laboratory of Molecular and Cellular Medicine, Department of Cellular and Physiological Sciences, Life Sciences Institute, The University of British Columbia, Vancouver, British Columbia, Canada tim.kieffer@ubc.ca.
Department of Surgery, The University of British Columbia, Vancouver, British Columbia, Canada.

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Classifications MeSH