Nitric Oxide Enhances Rice Resistance to Rice Black-Streaked Dwarf Virus Infection.
Nitric oxide
Osnia2 mutant rice
Rice
Rice black-streaked dwarf virus
Sodium nitroprusside
Journal
Rice (New York, N.Y.)
ISSN: 1939-8425
Titre abrégé: Rice (N Y)
Pays: United States
ID NLM: 101503136
Informations de publication
Date de publication:
14 Apr 2020
14 Apr 2020
Historique:
received:
28
10
2019
accepted:
12
03
2020
entrez:
16
4
2020
pubmed:
16
4
2020
medline:
16
4
2020
Statut:
epublish
Résumé
Rice black-streaked dwarf virus (RBSDV) causes one of the most important rice virus diseases of plants in East Asia. However, molecular mechanism(s)controlling rice resistance to infection is largely unknown. In this paper, we showed that RBSDV infection in rice significantly induced nitric oxide (NO) production. This finding was further validated through a genetic approach using a RBSDV susceptible (Nipponbare) and a RBSDV resistant (15HPO187) cultivar. The production of endogenous NO was muchhigher in the 15HPO187 plants, leading to a much lower RBSDV disease incidence. Pharmacological studies showed that the applications of NO-releasingcompounds (i.e., sodium nitroprusside [SNP] and nitrosoglutathione [GSNO]) to rice plants reduced RBSDV disease incidence. After RBSDV infection, the levels of OsICS1, OsPR1b and OsWRKY 45 transcripts were significantly up-regulated by NO in Nipponbare. The increased salicylic acid contents were also observed. After the SNP treatment, protein S-nitrosylation in rice plants was also increased, suggesting that the NO-triggered resistance to RBSDV infection was partially mediated at the post-translational level. Although Osnia2 mutant rice produced less endogenous NO after RBSDV inoculation and showed a higher RBSDV disease incidence, its RBSDV susceptibility could be reduced by SNP treatment. Collectively, our genetic and molecular evidence revealed that endogenous NO was a vital signal responsible for rice resistance to RBSDV infection.
Sections du résumé
BACKGROUND
BACKGROUND
Rice black-streaked dwarf virus (RBSDV) causes one of the most important rice virus diseases of plants in East Asia. However, molecular mechanism(s)controlling rice resistance to infection is largely unknown.
RESULTS
RESULTS
In this paper, we showed that RBSDV infection in rice significantly induced nitric oxide (NO) production. This finding was further validated through a genetic approach using a RBSDV susceptible (Nipponbare) and a RBSDV resistant (15HPO187) cultivar. The production of endogenous NO was muchhigher in the 15HPO187 plants, leading to a much lower RBSDV disease incidence. Pharmacological studies showed that the applications of NO-releasingcompounds (i.e., sodium nitroprusside [SNP] and nitrosoglutathione [GSNO]) to rice plants reduced RBSDV disease incidence. After RBSDV infection, the levels of OsICS1, OsPR1b and OsWRKY 45 transcripts were significantly up-regulated by NO in Nipponbare. The increased salicylic acid contents were also observed. After the SNP treatment, protein S-nitrosylation in rice plants was also increased, suggesting that the NO-triggered resistance to RBSDV infection was partially mediated at the post-translational level. Although Osnia2 mutant rice produced less endogenous NO after RBSDV inoculation and showed a higher RBSDV disease incidence, its RBSDV susceptibility could be reduced by SNP treatment.
CONCLUSIONS
CONCLUSIONS
Collectively, our genetic and molecular evidence revealed that endogenous NO was a vital signal responsible for rice resistance to RBSDV infection.
Identifiants
pubmed: 32291541
doi: 10.1186/s12284-020-00382-8
pii: 10.1186/s12284-020-00382-8
pmc: PMC7156532
doi:
Types de publication
Journal Article
Langues
eng
Pagination
24Subventions
Organisme : National Natural Science Foundation of China
ID : 31761143012
Organisme : National Natural Science Foundation of China
ID : 31601704
Organisme : Six talent peaks project of Jiangsu Province
ID : NY-056
Organisme : 333 high level talent training project of Jiangsu Province of Jiangxi Province (CN)
ID : BRA2018081
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