Coregistered Spectral Optical Coherence Tomography and Two-Photon Microscopy for Multimodal Near-Instantaneous Deep-Tissue Imaging.

in vivo imaging second harmonics generation spectral domain optical coherence tomography two-photon microscopy

Journal

Cytometry. Part A : the journal of the International Society for Analytical Cytology
ISSN: 1552-4930
Titre abrégé: Cytometry A
Pays: United States
ID NLM: 101235694

Informations de publication

Date de publication:
05 2020
Historique:
received: 10 12 2019
revised: 17 03 2020
accepted: 19 03 2020
pubmed: 16 4 2020
medline: 19 8 2021
entrez: 16 4 2020
Statut: ppublish

Résumé

Two-photon microscopy (2PM) has brought unique insight into the mechanisms underlying immune system dynamics and function since it enables monitoring of cellular motility and communication in complex systems within their genuine environment-the living organism. However, use of 2PM in clinical settings is limited. In contrast, optical coherence tomography (OCT), a noninvasive label-free diagnostic imaging method, which allows monitoring morphologic changes of large tissue regions in vivo, has found broad application in the clinic. Here we developed a combined multimodal technology to achieve near-instantaneous coregistered OCT, 2PM, and second harmonic generation (SHG) imaging over large volumes (up to 1,000 × 1,000 × 300 μm

Identifiants

pubmed: 32293804
doi: 10.1002/cyto.a.24012
doi:

Substances chimiques

Collagen 9007-34-5

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

515-527

Commentaires et corrections

Type : ErratumIn

Informations de copyright

© 2020 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry.

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Auteurs

Asylkhan Rakhymzhan (A)

Biophysical Analytics, Deutsches Rheumaforschungszentrum (DRFZ), Berlin, Germany.

Lucie Reuter (L)

Biophysical Analytics, Deutsches Rheumaforschungszentrum (DRFZ), Berlin, Germany.

Raphael Raspe (R)

Immundynamics, Deutsches Rheumaforschungszentrum (DRFZ), Berlin, Germany.
Immundynamics and Intravital Microscopy, Charité - Universitätsmedizin Berlin, Berlin, Germany.

Daniel Bremer (D)

Biophysical Analytics, Deutsches Rheumaforschungszentrum (DRFZ), Berlin, Germany.

Robert Günther (R)

Biophysical Analytics, Deutsches Rheumaforschungszentrum (DRFZ), Berlin, Germany.
Immundynamics, Deutsches Rheumaforschungszentrum (DRFZ), Berlin, Germany.

Ruth Leben (R)

Biophysical Analytics, Deutsches Rheumaforschungszentrum (DRFZ), Berlin, Germany.

Judith Heidelin (J)

LaVision BioTec-A Miltenyi Biotec Company, Bielefeld, Germany.

Volker Andresen (V)

LaVision BioTec-A Miltenyi Biotec Company, Bielefeld, Germany.

Sergey Cheremukhin (S)

LaVision BioTec-A Miltenyi Biotec Company, Bielefeld, Germany.

Hinnerk Schulz-Hildebrandt (H)

Institute of Biomedical Optics, University of Lübeck, Lübeck, Germany.

Maria G Bixel (MG)

Max-Plank-Institut for Molecular Biomedicine, Tissue Morphogenesis, Münster, Germany.

Ralf H Adams (RH)

Max-Plank-Institut for Molecular Biomedicine, Tissue Morphogenesis, Münster, Germany.

Helena Radbruch (H)

Institute for Neuropathology, Charité - Universitätsmedizin Berlin, Berlin, Germany.

Gereon Hüttmann (G)

Institute of Biomedical Optics, University of Lübeck, Lübeck, Germany.
Airway Research Center North (ARCN), Member of the German Center for Lung Research (DZL), Lübeck, Germany.

Anja E Hauser (AE)

Immundynamics, Deutsches Rheumaforschungszentrum (DRFZ), Berlin, Germany.
Immundynamics and Intravital Microscopy, Charité - Universitätsmedizin Berlin, Berlin, Germany.

Raluca A Niesner (RA)

Biophysical Analytics, Deutsches Rheumaforschungszentrum (DRFZ), Berlin, Germany.
Dynamic and Functional in vivo Imaging, Veterinary Medicine, Freie Universität Berlin, Berlin, Germany.

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