Bombyx mori nucleopolyhedrovirus Bm96 suppresses viral virulence in Bombyx mori larvae.
Baculovirus
Bm96
Bombyx mori
Enhanced locomotory activity
Killing speed
Journal
Journal of invertebrate pathology
ISSN: 1096-0805
Titre abrégé: J Invertebr Pathol
Pays: United States
ID NLM: 0014067
Informations de publication
Date de publication:
06 2020
06 2020
Historique:
received:
28
02
2020
accepted:
04
04
2020
pubmed:
16
4
2020
medline:
20
5
2021
entrez:
16
4
2020
Statut:
ppublish
Résumé
Bombyx mori nucleopolyhedrovirus (BmNPV) is a severe pathogen for the domestic silkworm, Bombyx mori. BmNPV harbors over 140 protein-coding genes in its 128.4 kilobase pair-long double-stranded genome. However, many BmNPV genes are still uncharacterized. Here we investigated the role of BmNPV Bm96 in both B. mori cultured cells and larvae. We found that Bm96 is mainly expressed at the late stage of infection and accumulation of Bm96 protein peaks at 24 h post infection (hpi) and declines gradually at 48 hpi in B. mori cultured cells. Compared with the wild-type viruses, Bm96-deletion viruses exhibited higher viral propagation and fast-killing phenotype in B. mori larvae. These results strongly suggest that Bm96 negatively regulates the propagation of BmNPV in B. mori larvae. Furthermore, we observed that larvae infected with Bm96-deletion viruses showed lower locomotory activity at the late stage of infection compared with those infected with the wild-type viruses.
Identifiants
pubmed: 32294464
pii: S0022-2011(20)30080-X
doi: 10.1016/j.jip.2020.107374
pii:
doi:
Substances chimiques
RNA, Viral
0
Viral Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
107374Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.