Adeno-Associated Virus Serotype 8-Mediated Genetic Labeling of Cholangiocytes in the Neonatal Murine Liver.
adeno-associated virus
cholangiocyte
liver
neonate
transduction
viral vector
Journal
Pharmaceutics
ISSN: 1999-4923
Titre abrégé: Pharmaceutics
Pays: Switzerland
ID NLM: 101534003
Informations de publication
Date de publication:
13 Apr 2020
13 Apr 2020
Historique:
received:
24
03
2020
revised:
08
04
2020
accepted:
10
04
2020
entrez:
17
4
2020
pubmed:
17
4
2020
medline:
17
4
2020
Statut:
epublish
Résumé
Determination of the cellular tropism of viral vectors is imperative for designing precise gene therapy. It has been widely accepted that transduction of hepatocytes using adeno-associated virus serotype 8 (AAV8) is a promising approach to correct inborn errors in neonates, but the type of neonatal hepatic cells transduced by AAV8 has not been thoroughly investigated. To address this question, we used a reporter mouse that carries Cre recombinase (Cre)-inducible yellow fluorescent protein (YFP). Our analysis primarily focused on cholangiocytes, given their pivotal roles in normal liver function and disease. We treated
Identifiants
pubmed: 32295003
pii: pharmaceutics12040351
doi: 10.3390/pharmaceutics12040351
pmc: PMC7238059
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : NCI NIH HHS
ID : R37 CA225807
Pays : United States
Organisme : NIH HHS
ID : R37 CA225807; T32 GM063483; P30 DK078392
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK078392
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM063483
Pays : United States
Organisme : Cincinnati Children's Research Foundation
ID : Trustee Award
Références
Mol Ther Methods Clin Dev. 2017 Dec 01;8:87-104
pubmed: 29326962
Dev Biol. 2015 Aug 15;404(2):136-48
pubmed: 26033091
PLoS One. 2017 Nov 15;12(11):e0187384
pubmed: 29140985
Genes Dev. 2016 Jul 15;30(14):1636-44
pubmed: 27445394
Discov Med. 2015 Jan;19(102):49-57
pubmed: 25636961
Cells Tissues Organs. 2017;203(4):243-257
pubmed: 27889769
Mol Ther. 2007 Sep;15(9):1677-85
pubmed: 17565352
Front Immunol. 2017 Nov 24;8:1604
pubmed: 29225598
PLoS One. 2013 Jun 25;8(6):e67680
pubmed: 23825679
Front Physiol. 2016 Feb 16;7:47
pubmed: 26909046
Am J Physiol Gastrointest Liver Physiol. 2013 Jan 1;304(1):G26-37
pubmed: 23104559
Gut Liver. 2012 Jul;6(3):295-304
pubmed: 22844556
Hum Gene Ther. 2012 May;23(5):533-9
pubmed: 22098408
Curr Opin Lipidol. 2018 Oct;29(5):417-422
pubmed: 30015675
Nat Methods. 2012 Jul;9(7):671-5
pubmed: 22930834
Hum Gene Ther. 2014 Sep;25(9):844-55
pubmed: 25072305
J Neurosci. 2005 Feb 9;25(6):1395-406
pubmed: 15703393
Cells. 2019 Apr 25;8(4):
pubmed: 31027317
J Clin Invest. 2015 Oct 1;125(10):3891-903
pubmed: 26348897
J Hepatol. 2019 Sep;71(3):573-585
pubmed: 31071368
Mol Ther. 2010 Jan;18(1):118-25
pubmed: 19861950
J Biol Chem. 2018 Jun 1;293(22):8656-8671
pubmed: 29666185
Int J Toxicol. 2014 May;33(3):219-237
pubmed: 24700568
Nat Biotechnol. 2016 Mar;34(3):334-8
pubmed: 26829317
BMC Dev Biol. 2001;1:4
pubmed: 11299042
World J Gastroenterol. 2016 Jan 28;22(4):1348-56
pubmed: 26819504
J Cell Sci. 2013 Nov 15;126(Pt 22):5239-46
pubmed: 24046446
Curr Gene Ther. 2005 Jun;5(3):323-31
pubmed: 15975009
Sci Transl Med. 2018 Nov 21;10(468):
pubmed: 30463916
Clin Vaccine Immunol. 2011 Sep;18(9):1586-8
pubmed: 21775517