Comparison of Methods for the Histological Evaluation of Odontocete Spiral Ganglion Cells.
cetaceans
decalcification
ear
histology
spiral ganglion
Journal
Animals : an open access journal from MDPI
ISSN: 2076-2615
Titre abrégé: Animals (Basel)
Pays: Switzerland
ID NLM: 101635614
Informations de publication
Date de publication:
14 Apr 2020
14 Apr 2020
Historique:
received:
01
03
2020
revised:
28
03
2020
accepted:
10
04
2020
entrez:
17
4
2020
pubmed:
17
4
2020
medline:
17
4
2020
Statut:
epublish
Résumé
Cetaceans greatly depend on their hearing system to perform many vital activities. The spiral ganglion is an essential component of the auditory pathway and can even be associated with injuries caused by anthropogenic noise. However, its anatomical location, characterized by surrounding bony structures, makes the anatomical and anatomopathological study of the spiral ganglion a difficult task. In order to obtain high-quality tissue samples, a perfect balance between decalcification and the preservation of neural components must be achieved. In this study, different methodologies for spiral ganglion sample preparation and preservation were evaluated. Hydrochloric acid had the shortest decalcification time but damaged the tissue extensively. Both formic acid and EDTA decalcification solutions had a longer decalcification time but exhibited better preservation of the neurons. However, improved cell morphology and staining were observed on ears pretreated with EDTA solution. Therefore, we suggest that decalcifying methodologies based on EDTA solutions should be used to obtain the highest quality samples for studying cell morphology and antigenicity in cetacean spiral ganglion neurons.
Identifiants
pubmed: 32295193
pii: ani10040683
doi: 10.3390/ani10040683
pmc: PMC7222732
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Agencia Canaria de Investigación, Innovación y Sociedad de la Información
ID : TESIS2015010113
Organisme : Ministerio de Economía y Competitividad
ID : PGC2018-101226-B-I00
Organisme : Macaronesian network for the transfer of interregional and multidisciplinary knowledge and technologies to protect, watch and monitor cetaceans and the marine environment, and analyse and exploit in a sustainable way the associated tourist activity
ID : MAC/1.1b/149
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