Serum albumin cysteine trioxidation is a potential oxidative stress biomarker of type 2 diabetes mellitus.
Aged
Biomarkers
/ blood
Case-Control Studies
Chromatography, High Pressure Liquid
/ methods
Cysteine
/ chemistry
Diabetes Mellitus, Type 2
/ blood
Disease Progression
Early Diagnosis
Female
Humans
Male
Middle Aged
Oxidation-Reduction
Oxidative Stress
Prognosis
Proteomics
/ methods
Serum Albumin, Human
/ analysis
Tandem Mass Spectrometry
/ methods
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
15 04 2020
15 04 2020
Historique:
received:
28
10
2019
accepted:
11
03
2020
entrez:
17
4
2020
pubmed:
17
4
2020
medline:
15
12
2020
Statut:
epublish
Résumé
Metabolic disorders in T2DM generate multiple sources of free radicals and oxidative stress that accelerate nonenzymatic degenerative protein modifications (DPMs) such as protein oxidation, disrupt redox signaling and physiological function, and remain a major risk factor for clinical diabetic vascular complications. In order to identify potential oxidative biomarkers in the blood plasma of patients with T2DM, we used LC-MS/MS-based proteomics to profile plasma samples from patients with T2DM and healthy controls. The results showed that human serum albumin (HSA) is damaged by irreversible cysteine trioxidation, which can be a potential oxidative stress biomarker for the early diagnosis of T2DM. The quantitative detection of site-specific thiol trioxidation is technically challenging; thus, we developed a sensitive and selective LC-MS/MS workflow that has been used to discover and quantify three unique thiol-trioxidized HSA peptides, ALVLIAFAQYLQQC
Identifiants
pubmed: 32296090
doi: 10.1038/s41598-020-62341-z
pii: 10.1038/s41598-020-62341-z
pmc: PMC7160123
doi:
Substances chimiques
Biomarkers
0
Cysteine
K848JZ4886
Serum Albumin, Human
ZIF514RVZR
Types de publication
Journal Article
Observational Study
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6475Références
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