The voltage-gated potassium channel Shaker promotes sleep via thermosensitive GABA transmission.
Activity Cycles
Animals
Animals, Genetically Modified
Behavior, Animal
Brain
/ metabolism
Circadian Rhythm
Dopaminergic Neurons
/ metabolism
Drosophila Proteins
/ genetics
Drosophila melanogaster
/ genetics
GABAergic Neurons
/ metabolism
Light
Receptors, GABA-A
/ genetics
Shaker Superfamily of Potassium Channels
/ genetics
Sleep
Synaptic Transmission
Thermosensing
Time Factors
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
15 04 2020
15 04 2020
Historique:
received:
20
05
2019
accepted:
20
03
2020
entrez:
17
4
2020
pubmed:
17
4
2020
medline:
16
6
2021
Statut:
epublish
Résumé
Genes and neural circuits coordinately regulate animal sleep. However, it remains elusive how these endogenous factors shape sleep upon environmental changes. Here, we demonstrate that Shaker (Sh)-expressing GABAergic neurons projecting onto dorsal fan-shaped body (dFSB) regulate temperature-adaptive sleep behaviors in Drosophila. Loss of Sh function suppressed sleep at low temperature whereas light and high temperature cooperatively gated Sh effects on sleep. Sh depletion in GABAergic neurons partially phenocopied Sh mutants. Furthermore, the ionotropic GABA receptor, Resistant to dieldrin (Rdl), in dFSB neurons acted downstream of Sh and antagonized its sleep-promoting effects. In fact, Rdl inhibited the intracellular cAMP signaling of constitutively active dopaminergic synapses onto dFSB at low temperature. High temperature silenced GABAergic synapses onto dFSB, thereby potentiating the wake-promoting dopamine transmission. We propose that temperature-dependent switching between these two synaptic transmission modalities may adaptively tune the neural property of dFSB neurons to temperature shifts and reorganize sleep architecture for animal fitness.
Identifiants
pubmed: 32296133
doi: 10.1038/s42003-020-0902-8
pii: 10.1038/s42003-020-0902-8
pmc: PMC7160125
doi:
Substances chimiques
Drosophila Proteins
0
Rdl protein, Drosophila
0
Receptors, GABA-A
0
Sh protein, Drosophila
0
Shaker Superfamily of Potassium Channels
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
174Références
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