Disrupting the LC3 Interaction Region (LIR) Binding of Selective Autophagy Receptors Sensitizes AML Cell Lines to Cytarabine.
AML – acute myeloid leukemia
LIR interaction
cytarabine
inhibitors
phage display
selective autophagy receptor
short linear motifs (SLiMs)
Journal
Frontiers in cell and developmental biology
ISSN: 2296-634X
Titre abrégé: Front Cell Dev Biol
Pays: Switzerland
ID NLM: 101630250
Informations de publication
Date de publication:
2020
2020
Historique:
received:
11
12
2019
accepted:
10
03
2020
entrez:
17
4
2020
pubmed:
17
4
2020
medline:
17
4
2020
Statut:
epublish
Résumé
Short linear motifs (SLiMs) located in disordered regions of multidomain proteins are important for the organization of protein-protein interaction networks. By dynamic association with their binding partners, SLiMs enable assembly of multiprotein complexes, pivotal for the regulation of various aspects of cell biology in higher organisms. Despite their importance, there is a paucity of molecular tools to study SLiMs of endogenous proteins in live cells. LC3 interacting regions (LIRs), being quintessential for orchestrating diverse stages of autophagy, are a prominent example of SLiMs and mediate binding to the ubiquitin-like LC3/GABARAP family of proteins. The role of LIRs ranges from the posttranslational processing of their binding partners at early stages of autophagy to the binding of selective autophagy receptors (SARs) to the autophagosome. In order to generate tools to study LIRs in cells, we engineered high affinity binders of LIR motifs of three archetypical SARs: OPTN, p62, and NDP52. In an array of
Identifiants
pubmed: 32296703
doi: 10.3389/fcell.2020.00208
pmc: PMC7137635
doi:
Types de publication
Journal Article
Langues
eng
Pagination
208Informations de copyright
Copyright © 2020 Putyrski, Vakhrusheva, Bonn, Guntur, Vorobyov, Brandts, Dikic and Ernst.
Références
PLoS One. 2011;6(4):e18556
pubmed: 21602908
Mol Cell. 2014 Jul 17;55(2):161-9
pubmed: 25038412
J Biol Chem. 2020 Apr 3;295(14):4526-4540
pubmed: 32086379
Biochem Biophys Res Commun. 2004 Jan 9;313(2):453-8
pubmed: 14684184
J Mol Biol. 2019 Nov 2;:
pubmed: 31682838
J Biol Chem. 2007 Aug 17;282(33):24131-45
pubmed: 17580304
J Clin Invest. 2009 May;119(5):1109-23
pubmed: 19363292
EMBO J. 2013 Jul 3;32(13):1903-16
pubmed: 23736261
Annu Rev Biochem. 2012;81:291-322
pubmed: 22482907
J Cell Sci. 2004 Jun 1;117(Pt 13):2805-12
pubmed: 15169837
J Cell Sci. 2013 Aug 1;126(Pt 15):3237-47
pubmed: 23908376
J Biol Chem. 2000 Aug 18;275(33):25445-50
pubmed: 10856287
Nature. 2007 Jun 21;447(7147):1021-5
pubmed: 17522630
Acta Crystallogr F Struct Biol Commun. 2017 Mar 1;73(Pt 3):130-137
pubmed: 28291748
J Cell Sci. 2014 Jan 1;127(Pt 1):3-9
pubmed: 24345374
Science. 2003 Apr 18;300(5618):445-52
pubmed: 12702867
Nat Cell Biol. 2014 Jun;16(6):495-501
pubmed: 24875736
Blood. 2016 Sep 1;128(9):1260-9
pubmed: 27268264
Int J Cancer. 1980 Aug;26(2):171-6
pubmed: 6970727
Nat Commun. 2019 May 3;10(1):2055
pubmed: 31053714
EMBO J. 2017 Feb 15;36(4):549-564
pubmed: 28028054
Int J Mol Sci. 2019 Jan 22;20(3):
pubmed: 30678185
Autophagy. 2013 May;9(5):784-6
pubmed: 23434839
Blood. 2016 Jan 7;127(1):71-8
pubmed: 26660428
Autophagy. 2018;14(1):66-79
pubmed: 29394115
Mol Cell Proteomics. 2012 Jul;11(7):M111.014969
pubmed: 22389433
Science. 2011 Jul 8;333(6039):228-33
pubmed: 21617041
Leukemia. 2019 Apr;33(4):981-994
pubmed: 30185934
Mol Cell. 2012 Nov 9;48(3):329-42
pubmed: 23022382
PLoS One. 2014 Apr 08;9(4):e94374
pubmed: 24714637
J Mol Biol. 2010 Jan 8;395(1):89-104
pubmed: 19854204
Blood. 2014 Jun 5;123(23):3622-34
pubmed: 24755409
ACS Chem Biol. 2008 Dec 19;3(12):749-55
pubmed: 19099396
J Mol Biol. 2000 Feb 18;296(2):487-95
pubmed: 10669603
Nat Commun. 2018 Oct 22;9(1):4373
pubmed: 30349045
Biochem J. 2013 Sep 15;454(3):459-66
pubmed: 23805866
Science. 2013 Feb 1;339(6119):590-5
pubmed: 23287719
Chem Rev. 2014 Jul 9;114(13):6733-78
pubmed: 24926813
Elife. 2017 Jun 15;6:
pubmed: 28617241
Sci Signal. 2009 Sep 08;2(87):ra50
pubmed: 19738200
PLoS Comput Biol. 2007 Feb 16;3(2):e25
pubmed: 17305419
J Biol Chem. 2017 Sep 15;292(37):15340-15351
pubmed: 28784659
Blood. 2019 Jan 10;133(2):168-179
pubmed: 30498063
Cell Rep. 2015 May 5;11(5):748-58
pubmed: 25921531
Structure. 2010 Oct 13;18(10):1233-43
pubmed: 20947012
Protein Sci. 2020 Feb;29(2):433-442
pubmed: 31654425
Mol Cell. 2019 Oct 17;76(2):268-285
pubmed: 31585693
Nature. 2015 Aug 20;524(7565):309-314
pubmed: 26266977
Autophagy. 2011 Mar;7(3):279-96
pubmed: 21189453