Investigation of in vivo anti-inflammatory and anti-angiogenic attributes of coumarin-rich ethanolic extract of Melilotus indicus.
Angiogenesis Inhibitors
/ administration & dosage
Animals
Anti-Inflammatory Agents
/ administration & dosage
Coumarins
/ isolation & purification
Disease Models, Animal
Dose-Response Relationship, Drug
Edema
/ drug therapy
Ethanol
/ chemistry
Female
Indomethacin
/ pharmacology
Inflammation
/ drug therapy
Lethal Dose 50
Melilotus
/ chemistry
Piroxicam
/ pharmacology
Plant Extracts
/ administration & dosage
Rats
Carrageenan
Formaldehyde
GC–MS
Histamine
Inflammation
Serotonin
Journal
Inflammopharmacology
ISSN: 1568-5608
Titre abrégé: Inflammopharmacology
Pays: Switzerland
ID NLM: 9112626
Informations de publication
Date de publication:
Feb 2021
Feb 2021
Historique:
received:
22
05
2019
accepted:
16
03
2020
pubmed:
17
4
2020
medline:
21
10
2021
entrez:
17
4
2020
Statut:
ppublish
Résumé
Inflammation and angiogenesis are two major contributors to tumourigenesis. Melilotus indicus is traditionally used as an anti-inflammatory agent. The current study was designed to investigate the anti-inflammatory and anti-angiogenic properties of ethanolic extract of M. indicus (Miet) whole plant and its marker compound (coumarin) using a series of in vivo methods. Extraction by maceration was adopted to prepare ethanolic extract. Phytochemical compounds present in Miet were investigated using both qualitative and quantitative methods. In vivo safety profile of Miet was investigated in behavioural studies. Four acute oedema models such as carrageenan, serotonin, histamine-induced paw oedema and xylene-induced ear oedema, and chronic formaldehyde-induced paw oedema model were employed to explore the anti-inflammatory potential of Miet. Chorioallantoic chick membrane assay (CAM) was performed to explore anti-angiogenic potential of Miet. Histopathological evaluations were conducted to access improvement in skin texture of paws. TNF-α ELISA kit was used to study effects of treatment on serum levels of TNF-α. Extraction by maceration resulted in formation of greenish coloured semisolid extract with a high coumarin content. In vivo toxicological studies revealed LD
Identifiants
pubmed: 32297069
doi: 10.1007/s10787-020-00703-9
pii: 10.1007/s10787-020-00703-9
doi:
Substances chimiques
Angiogenesis Inhibitors
0
Anti-Inflammatory Agents
0
Coumarins
0
Plant Extracts
0
Piroxicam
13T4O6VMAM
Ethanol
3K9958V90M
Indomethacin
XXE1CET956
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
281-293Subventions
Organisme : Higher Education Commission of Pakistan
ID : Research Grant No: 21-1828/SRGP/R&D/HEC/2018)
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