DNA Nanostructures for Targeted Antimicrobial Delivery.
Animals
Anti-Bacterial Agents
/ chemistry
Aptamers, Nucleotide
/ chemistry
Bacillus subtilis
/ chemistry
COS Cells
Chlorocebus aethiops
DNA
/ chemistry
Drug Carriers
/ chemistry
Enzymes, Immobilized
/ chemistry
Escherichia coli
/ chemistry
Microbial Sensitivity Tests
Muramidase
/ chemistry
Nanostructures
/ chemistry
Nucleic Acid Conformation
DNA nanostructures
antimicrobial
atomic force microscopy
bionanotechnology
dSTORM
Journal
Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Titre abrégé: Angew Chem Int Ed Engl
Pays: Germany
ID NLM: 0370543
Informations de publication
Date de publication:
27 07 2020
27 07 2020
Historique:
received:
21
02
2020
revised:
07
04
2020
pubmed:
17
4
2020
medline:
27
4
2021
entrez:
17
4
2020
Statut:
ppublish
Résumé
We report the use of DNA origami nanostructures, functionalized with aptamers, as a vehicle for delivering the antibacterial enzyme lysozyme in a specific and efficient manner. We test the system against Gram-positive (Bacillus subtilis) and Gram-negative (Escherichia coli) targets. We use direct stochastic optical reconstruction microscopy (dSTORM) and atomic force microscopy (AFM) to characterize the DNA origami nanostructures and structured illumination microscopy (SIM) to assess the binding of the origami to the bacteria. We show that treatment with lysozyme-functionalized origami slows bacterial growth more effectively than treatment with free lysozyme. Our study introduces DNA origami as a tool in the fight against antibiotic resistance, and our results demonstrate the specificity and efficiency of the nanostructure as a drug delivery vehicle.
Identifiants
pubmed: 32297692
doi: 10.1002/anie.202002740
pmc: PMC7496991
doi:
Substances chimiques
Anti-Bacterial Agents
0
Aptamers, Nucleotide
0
Drug Carriers
0
Enzymes, Immobilized
0
DNA
9007-49-2
Muramidase
EC 3.2.1.17
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
12698-12702Subventions
Organisme : Wellcome Trust
ID : 203249/Z/16/Z
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/K015850/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/K02292X/1
Pays : United Kingdom
Informations de copyright
© 2020 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.
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