Mind your assays: Misleading cytotoxicity with the WST-1 assay in the presence of manganese.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
07
11
2019
accepted:
27
03
2020
entrez:
17
4
2020
pubmed:
17
4
2020
medline:
17
7
2020
Statut:
epublish
Résumé
The WST-1 assay is the most common test to assess the in vitro cytotoxicity of chemicals. Tetrazolium-based assays can, however, be affected by the interference of tested chemicals, including carbon nanotubes or Mg particles. Here, we report a new interference of Mn materials with the WST-1 assay. Endothelial cells exposed to Mn particles (Mn alone or Fe-Mn alloy from 50 to 1600 μg/ml) were severely damaged according to the WST-1 assay, but not the ATP content assay. Subsequent experiments revealed that Mn particles interfere with the reduction of the tetrazolium salt to formazan. Therefore, the WST-1 assay is not suitable to evaluate the in vitro cytotoxicity of Mn-containing materials, and luminescence-based assays such as CellTiter-Glo® appear more appropriate.
Identifiants
pubmed: 32298350
doi: 10.1371/journal.pone.0231634
pii: PONE-D-19-31024
pmc: PMC7161962
doi:
Substances chimiques
Cytotoxins
0
Tetrazolium Salts
0
Manganese
42Z2K6ZL8P
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0231634Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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