Flow-controlled fluoroscopic angiography for the assessment of vascular integrity in bioengineered kidneys.
Angiography, Digital Subtraction
/ methods
Animals
Capillary Permeability
Feasibility Studies
Female
Fluoroscopy
/ methods
Graft Rejection
/ immunology
Humans
Kidney
/ blood supply
Kidney Transplantation
/ adverse effects
Reproducibility of Results
Sus scrofa
Tissue Engineering
/ methods
Tissue and Organ Harvesting
/ methods
Transplantation, Heterologous
/ methods
Transplantation, Homologous
/ methods
fluoroscopic angiography
kidney
organ transplantation
perfusion decellularization
vascular integrity
Journal
Artificial organs
ISSN: 1525-1594
Titre abrégé: Artif Organs
Pays: United States
ID NLM: 7802778
Informations de publication
Date de publication:
Oct 2020
Oct 2020
Historique:
received:
13
01
2020
revised:
06
03
2020
accepted:
09
04
2020
pubmed:
17
4
2020
medline:
12
10
2021
entrez:
17
4
2020
Statut:
ppublish
Résumé
Perfusion decellularization has been proposed as a promising method for generating nonimmunogenic organs from allogeneic or xenogeneic donors. Several imaging modalities have been used to assess vascular integrity in bioengineered organs with no consistency in the methodology used. Here, we studied the use of fluoroscopic angiography performed under controlled flow conditions for vascular integrity assessment in bioengineered kidneys. Porcine kidneys underwent ex vivo angiography before and after perfusion decellularization. Arterial and venous patencies were defined as visualization of contrast medium (CM) in distal capillaries and renal vein, respectively. Changes in vascular permeability were visualized and quantified. No differences in patency were detected in decellularized kidneys compared with native kidneys. However, focal parenchymal opacities and significant delay in CM clearance were detected in decellularized kidneys, indicating increased permeability. Biopsy-induced leakage was visualized in both groups, with digital subtraction angiography revealing minimal CM leakage earlier than nonsubtracted fluoroscopy. In summary, quantitative assessment of vascular permeability should be coupled with patency when studying the effect of perfusion decellularization on kidney vasculature. Flow-controlled angiography should be considered as the method of choice for vascular assessment in bioengineered kidneys. Adopting this methodology for organs premodified ex vivo under normothermic machine perfusion settings is also suggested.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1073-1080Informations de copyright
© 2020 International Center for Artificial Organs and Transplantation and Wiley Periodicals, Inc.
Références
U.S. Department of Health and Human Services. Available from: https://www.organdonor.gov/statistics-stories/statistics.html
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