In-flow measurement of cell-cell adhesion using oscillatory inertial microfluidics.
Journal
Lab on a chip
ISSN: 1473-0189
Titre abrégé: Lab Chip
Pays: England
ID NLM: 101128948
Informations de publication
Date de publication:
07 05 2020
07 05 2020
Historique:
pubmed:
18
4
2020
medline:
20
1
2021
entrez:
18
4
2020
Statut:
ppublish
Résumé
Multicellular clusters in circulation can exhibit a substantially different function and biomarker significance compared to individual cells. Notably, clusters of circulating tumor cells (CTCs) are much more effective initiators of metastasis than single CTCs, and correlate with worse patient prognoses. Measuring the cell-cell adhesion strength of CTC clusters is a critical step towards understanding their subsistence in the circulation and mechanism of elevated tumorigenicity. However, measuring cell-cell adhesion forces in flow is elusive using existing methods. Here, we report an oscillatory inertial microfluidics system which exerts a repeating fluidic force profile on suspended cell doublets to determine their cell-cell adhesion strength (F
Identifiants
pubmed: 32301448
doi: 10.1039/d0lc00089b
pmc: PMC7495683
mid: NIHMS1621775
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1612-1620Subventions
Organisme : NIBIB NIH HHS
ID : P41 EB002503
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA129933
Pays : United States
Organisme : NCI NIH HHS
ID : U01 CA214297
Pays : United States
Organisme : NIBIB NIH HHS
ID : U01 EB012493
Pays : United States
Références
Cell. 2014 Aug 28;158(5):1110-1122
pubmed: 25171411
J Biol Chem. 2004 May 7;279(19):19649-57
pubmed: 14990582
Cancer Res. 2018 Feb 15;78(4):845-852
pubmed: 29437766
Proc Natl Acad Sci U S A. 2018 Jul 24;115(30):7682-7687
pubmed: 29991599
Dev Biol. 2015 May 1;401(1):165-74
pubmed: 25448695
Nat Methods. 2008 Jun;5(6):491-505
pubmed: 18511917
Annu Rev Biochem. 2008;77:205-28
pubmed: 18307407
Anal Chem. 1998 Dec 1;70(23):4974-84
pubmed: 21644679
Science. 2011 Mar 25;331(6024):1559-64
pubmed: 21436443
Sci Transl Med. 2016 Jun 29;8(345):345ra89
pubmed: 27358499
Nature. 2014 Oct 16;514(7522):309-10
pubmed: 25318518
Nature. 2016 Sep 1;537(7618):102-106
pubmed: 27556950
Nat Rev Cancer. 2016 Apr;16(4):201-18
pubmed: 27009393
Biophys J. 1996 Mar;70(3):1529-33
pubmed: 8785310
Science. 2014 Jul 11;345(6193):216-20
pubmed: 25013076
Biophys J. 2000 Aug;79(2):707-19
pubmed: 10920005
Biophys J. 1999 Nov;77(5):2856-63
pubmed: 10545383
J Cell Biol. 2004 Dec 20;167(6):1183-94
pubmed: 15596540
Proc Natl Acad Sci U S A. 2016 Feb 16;113(7):E854-63
pubmed: 26831077
Eur J Cell Biol. 2000 Dec;79(12):975-81
pubmed: 11152288