Advanced Single-Cell Mapping Reveals that in hESC Cardiomyocytes Contraction Kinetics and Action Potential Are Independent of Myosin Isoform.

Action Potentials Cardiac Myosins / genetics Cell Differentiation Cells, Cultured Human Embryonic Stem Cells / cytology Humans Myocardial Contraction Myocytes, Cardiac / cytology Myosin Heavy Chains / genetics Protein Isoforms / genetics Single-Cell Analysis
MYH6 MYH7 action potential cardiac myosin heavy chain human embryonic stem cell-derived cardiomyocytes maturation single-cell mapping technique twitch contractions

Journal

Stem cell reports
ISSN: 2213-6711
Titre abrégé: Stem Cell Reports
Pays: United States
ID NLM: 101611300

Informations de publication

Date de publication:
12 05 2020
Historique:
received: 20 12 2018
revised: 12 03 2020
accepted: 17 03 2020
pubmed: 18 4 2020
medline: 17 4 2021
entrez: 18 4 2020
Statut: ppublish

Résumé

Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) represent an attractive model to investigate CM function and disease mechanisms. One characteristic marker of ventricular specificity of human CMs is expression of the ventricular, slow β-myosin heavy chain (MyHC), as opposed to the atrial, fast α-MyHC. The main aim of this study was to investigate at the single-cell level whether contraction kinetics and electrical activity of hESC-CMs are influenced by the relative expression of α-MyHC versus β-MyHC. For effective assignment of functional parameters to the expression of both MyHC isoforms at protein and mRNA levels in the very same hESC-CMs, we developed a single-cell mapping technique. Surprisingly, α- versus β-MyHC was not related to specific contractile or electrophysiological properties of the same cells. The multiparametric cell-by-cell analysis suggests that in hESC-CMs the expression of genes associated with electrical activity, contraction, calcium handling, and MyHCs is independently regulated.

Identifiants

DOI: 10.1016/j.stemcr.2020.03.015 PMID: 32302556 PMC: PMC7220955
pubmed: 32302556
pii: S2213-6711(20)30105-3
doi: 10.1016/j.stemcr.2020.03.015
pmc: PMC7220955
pii:
doi:

Substances chimiques

MYH6 protein, human 0
MYH7 protein, human 0
Protein Isoforms 0
Cardiac Myosins EC 3.6.1.-
Myosin Heavy Chains EC 3.6.4.1

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

788-802

Informations de copyright

Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.

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Auteurs

Natalie Weber (N)

Institute of Molecular and Cell Physiology, Hannover Medical School, Carl-Neuberg Straße 1, 30625 Hannover, Germany. Electronic address: weber.natalie@mh-hannover.de.

Kathrin Kowalski (K)

Institute of Molecular and Cell Physiology, Hannover Medical School, Carl-Neuberg Straße 1, 30625 Hannover, Germany.

Tim Holler (T)

Institute of Molecular and Cell Physiology, Hannover Medical School, Carl-Neuberg Straße 1, 30625 Hannover, Germany.

Ante Radocaj (A)

Institute of Molecular and Cell Physiology, Hannover Medical School, Carl-Neuberg Straße 1, 30625 Hannover, Germany.

Martin Fischer (M)

Institute of Neurophysiology, Hannover Medical School, Carl-Neuberg Straße 1, 30625 Hannover, Germany.

Stefan Thiemann (S)

Institute of Neurophysiology, Hannover Medical School, Carl-Neuberg Straße 1, 30625 Hannover, Germany.

Jeanne de la Roche (J)

Institute of Neurophysiology, Hannover Medical School, Carl-Neuberg Straße 1, 30625 Hannover, Germany.

Kristin Schwanke (K)

Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Carl-Neuberg Straße 1, 30625 Hannover, Germany.

Birgit Piep (B)

Institute of Molecular and Cell Physiology, Hannover Medical School, Carl-Neuberg Straße 1, 30625 Hannover, Germany.

Neele Peschel (N)

Institute of Molecular and Cell Physiology, Hannover Medical School, Carl-Neuberg Straße 1, 30625 Hannover, Germany.

Uwe Krumm (U)

Institute of Molecular and Cell Physiology, Hannover Medical School, Carl-Neuberg Straße 1, 30625 Hannover, Germany.

Alexander Lingk (A)

Institute of Molecular and Cell Physiology, Hannover Medical School, Carl-Neuberg Straße 1, 30625 Hannover, Germany.

Meike Wendland (M)

Institute of Molecular and Cell Physiology, Hannover Medical School, Carl-Neuberg Straße 1, 30625 Hannover, Germany.

Stephan Greten (S)

Institute of Molecular and Cell Physiology, Hannover Medical School, Carl-Neuberg Straße 1, 30625 Hannover, Germany.

Jan Dieter Schmitto (JD)

Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Carl-Neuberg Straße 1, 30625 Hannover, Germany.

Issam Ismail (I)

Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Carl-Neuberg Straße 1, 30625 Hannover, Germany.

Gregor Warnecke (G)

Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Carl-Neuberg Straße 1, 30625 Hannover, Germany.

Urs Zywietz (U)

Laser Zentrum Hannover e.V., Hollerithallee 8, 30419 Hannover, Germany.

Boris Chichkov (B)

Laser Zentrum Hannover e.V., Hollerithallee 8, 30419 Hannover, Germany.

Joachim Meißner (J)

Institute of Molecular and Cell Physiology, Hannover Medical School, Carl-Neuberg Straße 1, 30625 Hannover, Germany.

Axel Haverich (A)

Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Carl-Neuberg Straße 1, 30625 Hannover, Germany.

Ulrich Martin (U)

Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Carl-Neuberg Straße 1, 30625 Hannover, Germany.

Bernhard Brenner (B)

Institute of Molecular and Cell Physiology, Hannover Medical School, Carl-Neuberg Straße 1, 30625 Hannover, Germany.

Robert Zweigerdt (R)

Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Carl-Neuberg Straße 1, 30625 Hannover, Germany.

Theresia Kraft (T)

Institute of Molecular and Cell Physiology, Hannover Medical School, Carl-Neuberg Straße 1, 30625 Hannover, Germany.

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Classifications MeSH

questionsmedicales.fr › Phénomènes physiologiques de l'appareil locomoteur et du système nerveux › Phénomènes physiologiques du système nerveux › Potentiels de membrane › Potentiels d'action › Advanced Single-Cell Mapping Reveals that in...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines du cytosquelette › Protéines des microfilaments › Myosines › Myosine de type II › Myosines cardiaques › Advanced Single-Cell Mapping Reveals that in...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Différenciation cellulaire › Advanced Single-Cell Mapping Reveals that in...
questionsmedicales.fr › Cellules › Cellules cultivées › Advanced Single-Cell Mapping Reveals that in...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches pluripotentes › Cellules souches embryonnaires › Cellules souches embryonnaires humaines › Advanced Single-Cell Mapping Reveals that in...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Advanced Single-Cell Mapping Reveals that in...
questionsmedicales.fr › Phénomènes physiologiques de l'appareil locomoteur et du système nerveux › Phénomènes physiologiques du système locomoteur › Contraction musculaire › Contraction myocardique › Advanced Single-Cell Mapping Reveals that in...
questionsmedicales.fr › Cellules › Cellules musculaires › Myocytes cardiaques › Advanced Single-Cell Mapping Reveals that in...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines du cytosquelette › Protéines des microfilaments › Myosines › Chaînes lourdes de myosine › Advanced Single-Cell Mapping Reveals that in...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Isoformes de protéines › Advanced Single-Cell Mapping Reveals that in...
questionsmedicales.fr › Techniques d'investigation › Techniques cytologiques › Analyse sur cellule unique › Advanced Single-Cell Mapping Reveals that in...