Extracellular vesicles spread the RNA interference signal of Tribolium castaneum TcA cells.

Animals Cell Line Extracellular Vesicles / metabolism RNA Interference RNA, Double-Stranded / metabolism RNA, Small Interfering / metabolism Tribolium / physiology
Insect Intercellular communication RNAi Small RNA dsRNA siRNA

Journal

Insect biochemistry and molecular biology
ISSN: 1879-0240
Titre abrégé: Insect Biochem Mol Biol
Pays: England
ID NLM: 9207282

Informations de publication

Date de publication:
07 2020
Historique:
received: 18 07 2018
revised: 03 03 2020
accepted: 02 04 2020
pubmed: 18 4 2020
medline: 5 1 2021
entrez: 18 4 2020
Statut: ppublish

Résumé

The potential utility of RNA interference (RNAi) to control insect pests and viral infections depends largely on the target organism's ability to systemically spread the RNAi response. The efficacy of systemic RNAi varies among insects, though it has been shown to be high in the red flour beetle, Tribolium castaneum. We identified an extracellular RNAi signal that is present in the culture medium of T. castaneum (TcA) cells after treatment with long dsRNA specific for a luciferase reporter gene. Luciferase-specific siRNAs were detected in extracellular vesicles (EVs) that were purified from the culture medium of these dsRNA-treated cells. Furthermore, by measuring the silencing of luciferase expression, we showed that these siRNA-containing EVs can act as an RNAi signal for recipient TcA cells. We have therefore shown that a systemic RNAi response upon dsRNA treatment can be effectively spread through EVs.

Identifiants

DOI: 10.1016/j.ibmb.2020.103377 PMID: 32302638
pubmed: 32302638
pii: S0965-1748(20)30066-7
doi: 10.1016/j.ibmb.2020.103377
pii:
doi:

Substances chimiques

RNA, Double-Stranded 0
RNA, Small Interfering 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

103377

Informations de copyright

Copyright © 2020 Elsevier Ltd. All rights reserved.

Auteurs

Lina Mingels (L)

Molecular Developmental Physiology and Signal Transduction, Department of Biology, KU Leuven, 3000, Leuven, Belgium. Electronic address: lina.mingels@kuleuven.be.

Niels Wynant (N)

Molecular Developmental Physiology and Signal Transduction, Department of Biology, KU Leuven, 3000, Leuven, Belgium. Electronic address: niels.wynant@kuleuven.be.

Dulce Santos (D)

Molecular Developmental Physiology and Signal Transduction, Department of Biology, KU Leuven, 3000, Leuven, Belgium. Electronic address: dulce.cordeirodossantos@kuleuven.be.

Paulien Peeters (P)

Molecular Developmental Physiology and Signal Transduction, Department of Biology, KU Leuven, 3000, Leuven, Belgium. Electronic address: paulien.peeters@kuleuven.be.

Yannick Gansemans (Y)

Laboratory of Pharmaceutical Biotechnology, Gent University, 9000, Gent, Belgium. Electronic address: yannick.gansemans@ugent.be.

Johan Billen (J)

Socio-ecology and Social Evolution, Department of Biology, KU Leuven, 3000, Leuven, Belgium. Electronic address: johan.billen@kuleuven.be.

Filip Van Nieuwerburgh (F)

Laboratory of Pharmaceutical Biotechnology, Gent University, 9000, Gent, Belgium. Electronic address: filip.vannieuwerburgh@ugent.be.

Jozef Vanden Broeck (J)

Molecular Developmental Physiology and Signal Transduction, Department of Biology, KU Leuven, 3000, Leuven, Belgium. Electronic address: jozef.vandenbroeck@kuleuven.be.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Extracellular vesicles spread the RNA...
questionsmedicales.fr › Cellules › Cellules cultivées › Lignée cellulaire › Extracellular vesicles spread the RNA...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace extracellulaire › Vésicules extracellulaires › Extracellular vesicles spread the RNA...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Interférence par ARN › Extracellular vesicles spread the RNA...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Conformation d'acide nucléique › ARN double brin › Extracellular vesicles spread the RNA...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN non traduit › Petit ARN non traduit › Petit ARN interférent › Extracellular vesicles spread the RNA...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Arthropodes › Insectes › Pterygota › Neoptera › Holometabola › Coléoptères › Tribolium › Extracellular vesicles spread the RNA...