Interstitial fluid drainage from rat apical area takes place via vessels in the mandibular canal.
blood vessels
human serum albumin
lymph nodes
radioactivity
tooth apex
Journal
European journal of oral sciences
ISSN: 1600-0722
Titre abrégé: Eur J Oral Sci
Pays: England
ID NLM: 9504563
Informations de publication
Date de publication:
06 2020
06 2020
Historique:
accepted:
03
03
2020
pubmed:
20
4
2020
medline:
18
11
2020
entrez:
20
4
2020
Statut:
ppublish
Résumé
We sought to investigate the transport route for protein-rich fluid from the apical area towards the draining lymph nodes. The first mandibular molar root canals in 24 female Wistar rats were instrumented and filled with radioactive-labelled human serum albumin. The rats were sacrificed at different intervals beginning after 10 min (time 0) and continuing up to 72 h. Three jaw segments, gingiva around the first molar, blood samples, submandibular and cervical lymph nodes were collected and analyzed for radioactivity. The starting volume of tracer (control) for all experiments was calculated from measurements at time 0. At time 0, radioactivity was only detected in the jaw segments. Within lymph nodes and serum, the tracer was found after 4 h, with the highest amount recorded in serum up to 24 h. Lymphatics were found within the mandibular canal along blood vessels and nerves and exiting via foramen mandibularis, after immunohistochemical staining in four untreated rats. Our results show tracer distribution from the apical area towards the mandibular canal in a posterior direction. The tracer washout rate was low, and the fluid was mainly absorbed into blood vessels. The lymphatics in the mandibular canal may be more important for immune cell transport than for fluid drainage.
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
190-195Subventions
Organisme : Universitetet i Bergen
Pays : International
Organisme : Helse Vest
ID : 911684
Pays : International
Informations de copyright
© 2020 The Authors. Eur J Oral Sci published by John Wiley & Sons Ltd.
Références
Dahlen G. Microbiology and treatment of dental abscesses and periodontal-endodontic lesions. Periodontol 2000 2002; 28: 206-239.
Abbott PV. Classification, diagnosis and clinical manifestations of apical periodontitis. Endod Topics 2004; 8: 36-54.
Virtej A, Loes S, Iden O, Bletsa A, Berggreen E. Vascular endothelial growth factors signalling in normal human dental pulp: a study of gene and protein expression. Eur J Oral Sci 2013; 121: 92-100.
Martin A, Gasse H, Staszyk C. Absence of lymphatic vessels in the dog dental pulp: an immunohistochemical study. J Anat 2010; 217: 609-615.
Gerli R, Secciani I, Sozio F, Rossi A, Weber E, Lorenzini G. Absence of lymphatic vessels in human dental pulp: a morphological study. Eur J Oral Sci 2010; 118: 110-117.
Lohrberg M, Wilting J. The lymphatic vascular system of the mouse head. Cell Tissue Res 2016; 366: 667-677.
Bletsa A, Abdalla H, Loes S, Berggreen E. Lymphatic growth factors are expressed in human gingiva and upregulated in gingival fibroblasts after stimulation. J Periodontol 2018; 89: 606-615.
Bhingare AC, Ohno T, Tomura M, Zhang C, Aramaki O, Otsuki M, Tagami J, Azuma M. Dental pulp dendritic cells migrate to regional lymph nodes. J Dent Res 2014; 93: 288-293.
Kilkenny C, Browne WJ, Cuthill IC, Emerson M, Altman DG. Improving bioscience research reporting: the ARRIVE guidelines for reporting animal research. PLoS Biol 2010; 8: e1000412.
Wiig H, Tenstad O, Bert JL. Effect of hydration on interstitial distribution of charged albumin in rat dermis in vitro. J Physiol 2005; 569: 631-641.
Bletsa A, Virtej A, Berggreen E. Vascular endothelial growth factors and receptors are up-regulated during development of apical periodontitis. J Endod 2012; 38: 628-635.
Wiig H, Swartz MA. Interstitial fluid and lymph formation and transport: physiological regulation and roles in inflammation and cancer. Physiol Rev 2012; 92: 1005-1060.
Tenstad O, Heyeraas KJ, Wiig H, Aukland K. Drainage of plasma proteins from the renal medullary interstitium in rats. J Physiol 2001; 536: 533-539.
Yaghmaei M, Mashhadiabbas F, Shahabi S, Zafarbakhsh A, Yaghmaei S, Khojasteh A. Histologic evaluation of inferior alveolar lymphatics: an anatomic study. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2011; 112: 564-567.
Au B, Boulton MR, Narini PP, McCulloch CA, Hay JB. Lymph and interstitial fluid dynamics in labial gingival tissues of sheep. J Periodontal Res 1996; 31: 570-578.
Ando Y, Murai O, Kuwajima Y, Furukawa S, Sasaki D, Okawa Y, Yaegashi T, Miura H, Fujimura A. Lymphatic architecture of the human gingival interdental papilla. Lymphology 2011; 44: 146-154.
Goncalves PGP, Lourenco SIM, de Vasconcelos Gurgel BC. Immunohistochemical study of CD34 and podoplanin in periodontal disease. J Periodontal Res 2019; 54: 349-355.