Enhanced Adipose Mesenchymal Stem Cells Proliferation by Carboxymethyl-Chitosan Functionalized Polycaprolactone Nanofiber.
Animals
Calcium
/ metabolism
Cell Adhesion
/ drug effects
Cell Proliferation
/ drug effects
Cell Survival
/ drug effects
Chitosan
/ analogs & derivatives
Humans
Mesenchymal Stem Cells
/ cytology
Nanofibers
/ chemistry
Plasma Gases
/ pharmacology
Polyesters
/ pharmacology
Spectroscopy, Fourier Transform Infrared
Carboxymethyl chitosan
Mesenchymal stem cells
Tissue engineering
Journal
Iranian biomedical journal
ISSN: 2008-823X
Titre abrégé: Iran Biomed J
Pays: Iran
ID NLM: 9814853
Informations de publication
Date de publication:
07 2020
07 2020
Historique:
entrez:
21
4
2020
pubmed:
21
4
2020
medline:
5
2
2021
Statut:
ppublish
Résumé
Through combining two synthetic and natural polymers, scaffolds can be developed for tissue engineering and regenerative medicine purposes. In this work, carboxymethyl chitosan (CMC; 20%) was grafted to Polycaprolactone (PCL) nanofibers using the cold atmospheric plasma of helium. The PCL scaffolds were exposed to CAP, and functional groups were developed on the PCL surface. The results of Fourier Transform Infrared Spectroscopy confirmed CMC (20%) graft on PCL scaffold. The Thiazolyl blue tetrazolium bromide assay showed a significant enhancement (p < 0.05) in the cell affinity and proliferation of adipose-derived stem cells (ADSCs) to CMC20%-graft-PCL scaffolds. After 14 days, bone differentiation was affirmed through alizarin red and calcium depositions. Based on the results, the CMC20%-graft-PCL can support the proliferation of ADSCs and induce the differentiation into bone with longer culture time.
Sections du résumé
Background
Through combining two synthetic and natural polymers, scaffolds can be developed for tissue engineering and regenerative medicine purposes.
Methods
In this work, carboxymethyl chitosan (CMC; 20%) was grafted to Polycaprolactone (PCL) nanofibers using the cold atmospheric plasma of helium. The PCL scaffolds were exposed to CAP, and functional groups were developed on the PCL surface.
Results
The results of Fourier Transform Infrared Spectroscopy confirmed CMC (20%) graft on PCL scaffold. The Thiazolyl blue tetrazolium bromide assay showed a significant enhancement (p < 0.05) in the cell affinity and proliferation of adipose-derived stem cells (ADSCs) to CMC20%-graft-PCL scaffolds. After 14 days, bone differentiation was affirmed through alizarin red and calcium depositions.
Conclusion
Based on the results, the CMC20%-graft-PCL can support the proliferation of ADSCs and induce the differentiation into bone with longer culture time.
Identifiants
pubmed: 32306721
doi: 10.29252/ibj.24.4.236
pmc: PMC7275816
doi:
Substances chimiques
Plasma Gases
0
Polyesters
0
carboxymethyl-chitosan
0
polycaprolactone
24980-41-4
Chitosan
9012-76-4
Calcium
SY7Q814VUP
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
236-42Références
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